Epigenetic modifiers exert renal toxicity through induction of p66shc

Istvan Arany, Jeb S. Clark, Istvan Ember, Luis A. Juncos

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Abstract

Background/Aims: Trichostatin A (TSA) and 5-azacytidine (5AZA) induce reactive oxygen species (ROS)-mediated injury in renal proximal tubule cells. Since TSA and 5AZA are activators of p66shc, we questioned whether p66shc may mediate renal toxicity of TSA- and 5AZA. Materials and Methods: Renal proximal tubule cells were treated with either TSA or 5AZA for 24 hours followed by treatment with 200 μM H 2O 2. Expression of p66shc and activity of its promoter, as well as its mitochondrial and cytochrome c binding were determined. Impact of knockdown of p66shc and mutation of its cytochrome c-binding site on ROS production and cell injury was studied. Results: TSA, and 5AZA increased expression of p66shc through induction of its promoter and also increased its mitochondrial/cytochrome c binding. Knockdown or mutation of the cytochrome c binding site of p66shc attenuated ROS production and cell injury. Conclusion: Therapeutic means that interfere with induction of p66shc may ameliorate renal toxicity of those epigenetic modifiers.

Original languageEnglish
Pages (from-to)3267-3271
Number of pages5
JournalAnticancer research
Volume31
Issue number10
Publication statusPublished - Oct 1 2011

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Keywords

  • Epigenetic modifiers
  • Kidney
  • Mitochondria
  • P66shc
  • ROS
  • Toxicity

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Arany, I., Clark, J. S., Ember, I., & Juncos, L. A. (2011). Epigenetic modifiers exert renal toxicity through induction of p66shc. Anticancer research, 31(10), 3267-3271.