Ependymal cells variations in the central canal of the rat spinal cord flum terminale: An ultrastructural investigation

A. Mitro, K. Gallatz, M. Palkóvits, A. Kiss

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

objective. The ependymal cells, considered today as an active participant in neuroendocrine functions, were investigated by electron microscopy in the central canal of the lowest spinal cord, the flum terminale (FT), in adult rats. In this area of the spinal cord, the central canal is covered by a heterogeneous population of ependymal cells. The aim of the present work was to compare the regional features of the ependymal cells in two different parts of the FT with a special regard to their ultrastructure. Methods. Two parts of the FT were selected for the ultrastructural observations: the rostral (rFT) and the caudal (cFT) ones. The rTF was removed at the level of the immediate continuation of the conus medullaris, while the cFT 30 mm further caudally. Afer formaldehyde fxation, the spinal cord was removed and cut into small blocks for electron microscopic processing. The material was embedded into durcupan, contrasted with uranyl acetate, lead citrate as well as osmium tetroxide, and investigated under JEOL 1200 EX electron microscope. results. In the rFT, the ependymal lining is pseudostratifed and one-layered in the cFT, whereas the shape of the ependymal cells may vary from cuboidal to fatten in the rostro-caudal direction. The basal membrane of many ependymal cells possesses deep invaginations, so called "flum terminale labyrinths". Many neuronal processes occur in the pericanalicular neuropil. In contrast to the rFT, the cFT is less rich in the neuropil particles. Some of the ependymal cells concurrently reach both the intracanalicular and extracanalicular cerebrospinal fuid (CSF), thus they may represent a new variant of the ependymal cells designated as "bridge cells of the FT". Conclusions. The present data indicate that the FT ependymal cells exhibit clear differences in anatomy as well as ultrastructure that may reflect their distinct functional activity. Therefore, observations presented here may serve for the better understanding of the physiological role of the individual ependymal areas in this special portion of the rat spinal cord.

Original languageEnglish
Pages (from-to)93-99
Number of pages7
JournalEndocrine Regulations
Volume47
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

Spinal Cord
Neuropil
Electrons
Osmium Tetroxide
Cell Shape
Inner Ear
Citric Acid
Formaldehyde
Anatomy
Electron Microscopy
Membranes
Population

Keywords

  • Electron microscopy
  • Ependyma
  • Rat
  • Spinal cord flum terminale

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ependymal cells variations in the central canal of the rat spinal cord flum terminale : An ultrastructural investigation. / Mitro, A.; Gallatz, K.; Palkóvits, M.; Kiss, A.

In: Endocrine Regulations, Vol. 47, No. 2, 2013, p. 93-99.

Research output: Contribution to journalArticle

@article{aba22f31a4c64eb4ae0838fe967ec0c9,
title = "Ependymal cells variations in the central canal of the rat spinal cord flum terminale: An ultrastructural investigation",
abstract = "objective. The ependymal cells, considered today as an active participant in neuroendocrine functions, were investigated by electron microscopy in the central canal of the lowest spinal cord, the flum terminale (FT), in adult rats. In this area of the spinal cord, the central canal is covered by a heterogeneous population of ependymal cells. The aim of the present work was to compare the regional features of the ependymal cells in two different parts of the FT with a special regard to their ultrastructure. Methods. Two parts of the FT were selected for the ultrastructural observations: the rostral (rFT) and the caudal (cFT) ones. The rTF was removed at the level of the immediate continuation of the conus medullaris, while the cFT 30 mm further caudally. Afer formaldehyde fxation, the spinal cord was removed and cut into small blocks for electron microscopic processing. The material was embedded into durcupan, contrasted with uranyl acetate, lead citrate as well as osmium tetroxide, and investigated under JEOL 1200 EX electron microscope. results. In the rFT, the ependymal lining is pseudostratifed and one-layered in the cFT, whereas the shape of the ependymal cells may vary from cuboidal to fatten in the rostro-caudal direction. The basal membrane of many ependymal cells possesses deep invaginations, so called {"}flum terminale labyrinths{"}. Many neuronal processes occur in the pericanalicular neuropil. In contrast to the rFT, the cFT is less rich in the neuropil particles. Some of the ependymal cells concurrently reach both the intracanalicular and extracanalicular cerebrospinal fuid (CSF), thus they may represent a new variant of the ependymal cells designated as {"}bridge cells of the FT{"}. Conclusions. The present data indicate that the FT ependymal cells exhibit clear differences in anatomy as well as ultrastructure that may reflect their distinct functional activity. Therefore, observations presented here may serve for the better understanding of the physiological role of the individual ependymal areas in this special portion of the rat spinal cord.",
keywords = "Electron microscopy, Ependyma, Rat, Spinal cord flum terminale",
author = "A. Mitro and K. Gallatz and M. Palk{\'o}vits and A. Kiss",
year = "2013",
doi = "10.4149/endo_20130293",
language = "English",
volume = "47",
pages = "93--99",
journal = "Endocrine Regulations",
issn = "1210-0668",
publisher = "Institute of Experimental Endocrinology",
number = "2",

}

TY - JOUR

T1 - Ependymal cells variations in the central canal of the rat spinal cord flum terminale

T2 - An ultrastructural investigation

AU - Mitro, A.

AU - Gallatz, K.

AU - Palkóvits, M.

AU - Kiss, A.

PY - 2013

Y1 - 2013

N2 - objective. The ependymal cells, considered today as an active participant in neuroendocrine functions, were investigated by electron microscopy in the central canal of the lowest spinal cord, the flum terminale (FT), in adult rats. In this area of the spinal cord, the central canal is covered by a heterogeneous population of ependymal cells. The aim of the present work was to compare the regional features of the ependymal cells in two different parts of the FT with a special regard to their ultrastructure. Methods. Two parts of the FT were selected for the ultrastructural observations: the rostral (rFT) and the caudal (cFT) ones. The rTF was removed at the level of the immediate continuation of the conus medullaris, while the cFT 30 mm further caudally. Afer formaldehyde fxation, the spinal cord was removed and cut into small blocks for electron microscopic processing. The material was embedded into durcupan, contrasted with uranyl acetate, lead citrate as well as osmium tetroxide, and investigated under JEOL 1200 EX electron microscope. results. In the rFT, the ependymal lining is pseudostratifed and one-layered in the cFT, whereas the shape of the ependymal cells may vary from cuboidal to fatten in the rostro-caudal direction. The basal membrane of many ependymal cells possesses deep invaginations, so called "flum terminale labyrinths". Many neuronal processes occur in the pericanalicular neuropil. In contrast to the rFT, the cFT is less rich in the neuropil particles. Some of the ependymal cells concurrently reach both the intracanalicular and extracanalicular cerebrospinal fuid (CSF), thus they may represent a new variant of the ependymal cells designated as "bridge cells of the FT". Conclusions. The present data indicate that the FT ependymal cells exhibit clear differences in anatomy as well as ultrastructure that may reflect their distinct functional activity. Therefore, observations presented here may serve for the better understanding of the physiological role of the individual ependymal areas in this special portion of the rat spinal cord.

AB - objective. The ependymal cells, considered today as an active participant in neuroendocrine functions, were investigated by electron microscopy in the central canal of the lowest spinal cord, the flum terminale (FT), in adult rats. In this area of the spinal cord, the central canal is covered by a heterogeneous population of ependymal cells. The aim of the present work was to compare the regional features of the ependymal cells in two different parts of the FT with a special regard to their ultrastructure. Methods. Two parts of the FT were selected for the ultrastructural observations: the rostral (rFT) and the caudal (cFT) ones. The rTF was removed at the level of the immediate continuation of the conus medullaris, while the cFT 30 mm further caudally. Afer formaldehyde fxation, the spinal cord was removed and cut into small blocks for electron microscopic processing. The material was embedded into durcupan, contrasted with uranyl acetate, lead citrate as well as osmium tetroxide, and investigated under JEOL 1200 EX electron microscope. results. In the rFT, the ependymal lining is pseudostratifed and one-layered in the cFT, whereas the shape of the ependymal cells may vary from cuboidal to fatten in the rostro-caudal direction. The basal membrane of many ependymal cells possesses deep invaginations, so called "flum terminale labyrinths". Many neuronal processes occur in the pericanalicular neuropil. In contrast to the rFT, the cFT is less rich in the neuropil particles. Some of the ependymal cells concurrently reach both the intracanalicular and extracanalicular cerebrospinal fuid (CSF), thus they may represent a new variant of the ependymal cells designated as "bridge cells of the FT". Conclusions. The present data indicate that the FT ependymal cells exhibit clear differences in anatomy as well as ultrastructure that may reflect their distinct functional activity. Therefore, observations presented here may serve for the better understanding of the physiological role of the individual ependymal areas in this special portion of the rat spinal cord.

KW - Electron microscopy

KW - Ependyma

KW - Rat

KW - Spinal cord flum terminale

UR - http://www.scopus.com/inward/record.url?scp=84878380942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878380942&partnerID=8YFLogxK

U2 - 10.4149/endo_20130293

DO - 10.4149/endo_20130293

M3 - Article

C2 - 23641790

AN - SCOPUS:84878380942

VL - 47

SP - 93

EP - 99

JO - Endocrine Regulations

JF - Endocrine Regulations

SN - 1210-0668

IS - 2

ER -