Enzymic methylation of ascorbigen and demethylation of its n-methyl derivative by pseudomonas savastanoi pv. phaseolicola bacteria

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Abstract

Biotransformation of ascorbigen and N-methylascorbigen as a model compound pair was studied in the presence of Pseudomonas savastanoi pv. phaseolicola bacterium cells. These compounds were separated from a methanolic mixture by using an overpressured layer chromatographic method. After saturation of the developed chromatoplates with the bacterial cell suspension, the composition of the chromatographic spots was analyzed by means of high performance liquid chromatography. Pattern recognition by independent multicategory analysis was used to compare of the retention behavior of the standard and putative ascorbigen and methylascorbigen, and to manage the analytical data. Results showed that ascorbigen was partially methylated and N-methylascorbigen was partially demethylated by the bacteria. Our new results confirm the former assumption that formaldehyde is formed by demethylation of the N-methyl group of methylascorbigen.

Original languageEnglish
Pages (from-to)1001-1007
Number of pages7
JournalChromatographia
Volume75
Issue number17-18
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Methylation
Pseudomonas
Bacteria
Derivatives
High performance liquid chromatography
Biotransformation
Formaldehyde
Pattern recognition
Suspensions
High Pressure Liquid Chromatography
Chemical analysis
ascorbigen

Keywords

  • Ascorbigen
  • Enzymic methylation-demethylation
  • Formaldehyde
  • Mechanism
  • Methylascorbigen
  • Overpressured layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

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title = "Enzymic methylation of ascorbigen and demethylation of its n-methyl derivative by pseudomonas savastanoi pv. phaseolicola bacteria",
abstract = "Biotransformation of ascorbigen and N-methylascorbigen as a model compound pair was studied in the presence of Pseudomonas savastanoi pv. phaseolicola bacterium cells. These compounds were separated from a methanolic mixture by using an overpressured layer chromatographic method. After saturation of the developed chromatoplates with the bacterial cell suspension, the composition of the chromatographic spots was analyzed by means of high performance liquid chromatography. Pattern recognition by independent multicategory analysis was used to compare of the retention behavior of the standard and putative ascorbigen and methylascorbigen, and to manage the analytical data. Results showed that ascorbigen was partially methylated and N-methylascorbigen was partially demethylated by the bacteria. Our new results confirm the former assumption that formaldehyde is formed by demethylation of the N-methyl group of methylascorbigen.",
keywords = "Ascorbigen, Enzymic methylation-demethylation, Formaldehyde, Mechanism, Methylascorbigen, Overpressured layer chromatography",
author = "Edith K{\'a}tay and Z. N{\'e}meth and P. Ott and E. Tyih{\'a}k and G. K{\'a}tay",
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T1 - Enzymic methylation of ascorbigen and demethylation of its n-methyl derivative by pseudomonas savastanoi pv. phaseolicola bacteria

AU - Kátay, Edith

AU - Németh, Z.

AU - Ott, P.

AU - Tyihák, E.

AU - Kátay, G.

PY - 2012/9

Y1 - 2012/9

N2 - Biotransformation of ascorbigen and N-methylascorbigen as a model compound pair was studied in the presence of Pseudomonas savastanoi pv. phaseolicola bacterium cells. These compounds were separated from a methanolic mixture by using an overpressured layer chromatographic method. After saturation of the developed chromatoplates with the bacterial cell suspension, the composition of the chromatographic spots was analyzed by means of high performance liquid chromatography. Pattern recognition by independent multicategory analysis was used to compare of the retention behavior of the standard and putative ascorbigen and methylascorbigen, and to manage the analytical data. Results showed that ascorbigen was partially methylated and N-methylascorbigen was partially demethylated by the bacteria. Our new results confirm the former assumption that formaldehyde is formed by demethylation of the N-methyl group of methylascorbigen.

AB - Biotransformation of ascorbigen and N-methylascorbigen as a model compound pair was studied in the presence of Pseudomonas savastanoi pv. phaseolicola bacterium cells. These compounds were separated from a methanolic mixture by using an overpressured layer chromatographic method. After saturation of the developed chromatoplates with the bacterial cell suspension, the composition of the chromatographic spots was analyzed by means of high performance liquid chromatography. Pattern recognition by independent multicategory analysis was used to compare of the retention behavior of the standard and putative ascorbigen and methylascorbigen, and to manage the analytical data. Results showed that ascorbigen was partially methylated and N-methylascorbigen was partially demethylated by the bacteria. Our new results confirm the former assumption that formaldehyde is formed by demethylation of the N-methyl group of methylascorbigen.

KW - Ascorbigen

KW - Enzymic methylation-demethylation

KW - Formaldehyde

KW - Mechanism

KW - Methylascorbigen

KW - Overpressured layer chromatography

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