Enzymic methods for enrichment of fungal mutants I. Enrichment of Schizosaccharomyces pombe mutants

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Abstract

A simple and widely applicable technique has been elaborated for enrichment of auxotrophic and thermosensitive mutants of Schizosaccharomyces pombe. The method is based on the selective action of cell-wall lytic enzymes from the digestive juice of Helix pomatia and of enzymes from Trichoderma viride. Under conditions where wild-type cells in mixed cultures of old wild-type and mutant cells are selectively induced to grow, the wild-type cells can be lysed and eliminated. The efficiency of the method was increased by using 2-deoxyglucose, an inhibitor of cell-wall sythesis. Cells of Schizossacharomyces pombe, growing in a medium containing glucose and 2-deoxyglucose at a ratio of 40 : 1, were highly sensitive to the lytic effects of enzymes from Helix pomatia digestive juice and from Trichoderma viride.

Original languageEnglish
Pages (from-to)163-173
Number of pages11
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume50
Issue number2
DOIs
Publication statusPublished - 1978

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Schizosaccharomyces
Trichoderma
Deoxyglucose
Cell Wall
Enzymes
Glucose

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

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abstract = "A simple and widely applicable technique has been elaborated for enrichment of auxotrophic and thermosensitive mutants of Schizosaccharomyces pombe. The method is based on the selective action of cell-wall lytic enzymes from the digestive juice of Helix pomatia and of enzymes from Trichoderma viride. Under conditions where wild-type cells in mixed cultures of old wild-type and mutant cells are selectively induced to grow, the wild-type cells can be lysed and eliminated. The efficiency of the method was increased by using 2-deoxyglucose, an inhibitor of cell-wall sythesis. Cells of Schizossacharomyces pombe, growing in a medium containing glucose and 2-deoxyglucose at a ratio of 40 : 1, were highly sensitive to the lytic effects of enzymes from Helix pomatia digestive juice and from Trichoderma viride.",
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AU - Ferenczy, L.

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AB - A simple and widely applicable technique has been elaborated for enrichment of auxotrophic and thermosensitive mutants of Schizosaccharomyces pombe. The method is based on the selective action of cell-wall lytic enzymes from the digestive juice of Helix pomatia and of enzymes from Trichoderma viride. Under conditions where wild-type cells in mixed cultures of old wild-type and mutant cells are selectively induced to grow, the wild-type cells can be lysed and eliminated. The efficiency of the method was increased by using 2-deoxyglucose, an inhibitor of cell-wall sythesis. Cells of Schizossacharomyces pombe, growing in a medium containing glucose and 2-deoxyglucose at a ratio of 40 : 1, were highly sensitive to the lytic effects of enzymes from Helix pomatia digestive juice and from Trichoderma viride.

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