Enzymes in normally perfused and ischaemic dog hearts which release a substance with kinin like activity

I. J. Zeitlin, S. O. Fagbemi, J. R. Parratt

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

The presence of amidases cleaving the tripeptide VAL.LEU.ARG.PNA, and liberating from human plasma kininogen substance(s) able to contract uterine smooth muscle and to lower blood pressure (uterus contracting and hypotensive activity), has been demonstrated in vascular and muscle tissues from normally perfused and ischaemic areas of dog hearts. Studies were carried out on blood free preparations of coronary arteries and veins and normally perfused and ischaemic ventricle. All the tissues were found to contain both acid optimum (pH 6) and alkaline optimum (pH> 9) enzymes forming uterus contracting substance (UCS, bioassayed on isolated uterus of rats in oestrus), the highest levels of both activities being found in arterial tissues and the least in ventricle. Enzyme levels in ischaemic or normally perfused ventricle did not differ significantly. Gel filtration (Sephacryl, S-300) of coronary artery extracts gave one peak each of acid optimum enzyme with a molecular weight of 38 300±800 daltons and alkaline optimum enzyme with a molecular weight of 92 100±4000 daltons. Both acid and alkaline enzyme fractions cleaved the tripeptide substrate with pH optima identical to those for UCS formation. The acid optimum activity, both of UCS formation and tripeptide cleavage, was inhibited by pepstatin but not by aprotinin or soybean trypsin inhibitor (SBTI). The alkaline optimum activity was inhibited by aprotinin and SBTI but not pepstatin. Both acid and alkaline optimum enzymes released a hypotensive agent from a plasma protein substrate. The molecular weight and response to inhibitors of the acid optimum enzyme were similar to a cathepsin, and those of the alkaline optimum enzyme were similar to plasma kallikrein.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalCardiovascular research
Volume23
Issue number2
DOIs
Publication statusPublished - Feb 1989

Keywords

  • Cathepsin
  • Heart
  • Kallikrein
  • Kinin like peptide
  • Vasoactive peptides

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Enzymes in normally perfused and ischaemic dog hearts which release a substance with kinin like activity'. Together they form a unique fingerprint.

  • Cite this