Enzyme production of the edible mushroom pleorotus ostreatus in shaken cultures completed with agro-industrial wastes

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Abstract

The enzyme production of the white-rot fungus, the edible mushroom Pleurotus ostreatus, was determined in shaken culture media containing extracts of agro-industrial wastes (tomato, potato and pepper residues) as an unique carbon source. The activity of β-glucosidase, xylanase, laccase as well as manganese-dependent and independent peroxidases was measured at 0, 3.5, 7.0, 10.5, 14.0, 17.5, 21.0, 24.5, 28.0 and 31.5 days of cultivation. A spectral mapping technique and non-linear mapping were employed for the calculation of the relationships among the fermentation parameters, such as fermentation time, enzyme activity and selectivity of enzyme production. It was established that P. ostreatus produced β-glucosidase, xylanase, laccase, manganese-dependent and independent peroxidases in each culture medium and that the enzyme activities were higher in cultures containing agro-industrial wastes than in the control containing glucose as a carbon source. The spectral mapping technique allowed demonstrating that the enzyme activities were the highest in the culture completed with pepper extract followed by cultures containing potato and tomato extracts. The differences among the selectivity of the enzyme activities were negligible up to 21.0 days of fermentation and reached the maximum at the end of the fermentation process. The production of laccase as well as manganese-dependent and independent peroxidases showed similar patterns while the selectivity patterns of xylanase and β-galactoside production were different. In addition, it became evident that the agro-industrial wastes influenced the enzyme production in a distinct way.

Original languageEnglish
Pages (from-to)152-157
Number of pages6
JournalEngineering in Life Sciences
Volume5
Issue number2
DOIs
Publication statusPublished - Apr 1 2005

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Bioengineering

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