Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE

Maria Kávai, Aniko Bányai, Attila Zsindely, Ildiko Sonkoly, G. Szegedi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A micro-ELISA technique has been developed to measure antibodies to native DNA and used in SLE patients. The distribution of antibody to native DNA in the main immunoglobulin classes was studied, using anti-human globulin conjugates labelled with peroxidase. The antigen (double-stranded DNA from calf thymus) used in the assay was absorbed to the surface of polystyrene plates treated with methylated bovine serum albumin. The standardization of the method was carried out by use of globulin calibration curves.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalJournal of Immunological Methods
Volume48
Issue number2
DOIs
Publication statusPublished - Jan 29 1982

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Enzyme-Linked Immunosorbent Assay
Globulins
Antibodies
DNA
Serum
Immunoglobulin Isotypes
Polystyrenes
Thymus Gland
Calibration
Peroxidase
Antigens
methylated bovine serum albumin

Keywords

  • anti-native DNA
  • micro-ELISA
  • SLE

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE. / Kávai, Maria; Bányai, Aniko; Zsindely, Attila; Sonkoly, Ildiko; Szegedi, G.

In: Journal of Immunological Methods, Vol. 48, No. 2, 29.01.1982, p. 169-175.

Research output: Contribution to journalArticle

Kávai, Maria ; Bányai, Aniko ; Zsindely, Attila ; Sonkoly, Ildiko ; Szegedi, G. / Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE. In: Journal of Immunological Methods. 1982 ; Vol. 48, No. 2. pp. 169-175.
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