Enzyme electrodes for the determination of 1 -phenylalanine

G. G. Guilbault, G. Nagy

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Two different kinds of enzyme electrodes suitable for the determination of 1-phenylalanine are described. The 1-phenylalanine electrode built based on an ion-selective iodide electrode uses a dual enzyme reaction layer 1-amino acid oxidase (1-AAO), horseradish peroxidase (HRP) - chemically immobilized in polyacryl gel. The electrode function is based on the reaction: The constant I-concentration of the sample solution decreases locally at the electrode surface in the presence of 1-phenylalanine (l-PA). The 1-phenylalanine electrode built on a silicone rubber based antibiotic type ammonium selective electrode was fabricated with a chemically immobilized 1-amino acid oxidase, as reaction layer. The 1-phenylalanine diffuses into the enzyme layer where it is converted to NH4 +ions which are sensed by the antibiotic membrane which is selective for NH4 + ions. A potential is produced which is a log function of the concentration of 1-phenylalanine.

Original languageEnglish
Pages (from-to)301-312
Number of pages12
JournalAnalytical Letters
Volume6
Issue number4
DOIs
Publication statusPublished - 1973

Keywords

  • 1-phenylalanine
  • ammonium electrode
  • enzyme electrode
  • iodide sensor

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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