Enzyme Based Amperometric Biosensor for Adenine Determination

Beáta Bóka, Nóra Adányi, Diána Virág, Ivo Frebort, Attila Kiss

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An enzyme-based amperometric biosensor was developed for the rapid determination of adenine in biological and food samples. Recombinant adenine deaminase (EC 3.5.4.2) from Schizosaccharomyces pombe was co-immobilized on graphite electrode with xanthine oxidase (EC 1.17.3.2), peroxidase (EC 1.11.1.7) and an Os mediator. Optimal working conditions were pH7.0 and -150mV vs. Ag/AgCl. The novel method was characterized by a detection limit of 1μM and a linear measuring range of 0.005-0.1mM. Different beer and tea samples were analyzed by the newly elaborated adenine biosensor. The results obtained correlate with the adenine content measured by the reference HPLC method.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalElectroanalysis
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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Keywords

  • Adenine
  • Adenine deaminase
  • Biosensors
  • Electrochemistry
  • Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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