Enzyme activity changes in rat heart after chronic alcohol ingestion

I. Édes, Ágota Andó, Miklós Csanády, Hortenzia Mazareán, Ferenc Guba

Research output: Contribution to journalReview article

12 Citations (Scopus)

Abstract

Summary: The effect of a chronic intake of dietary alcohol upon myocardial enzymes was studied in rats. Alcohol, comprising more than 40% of the dietary calorie content, was administered to rats for 6 or 12 weeks. To assess the metabolic changes in the myocardium, the following enzymes were measured: lactate dehydrogenase (LDH), malate dehydrogenase (MDH), aldolase (ALD), isocitrate dehydrogenase (ICDH), creatine kinase (CK) and glutamate-pyruvate transaminase (GPT).The activity of CK was decreased (4.79±0.99 U·mg-1 protein) after 6 weeks on alcohol and was significantly different from that of the controls (5.98±1.44 U·mg-1 protein). After 12 weeks the CK activity of alcoholic rats had recovered to 5.99±1.08 U·mg protein-1 and approached the value found in the normal myocardium. A pronounced decrease was found in the activity of MDH: 8.26±0.69 U·mg protein-1 in the controls, and 6.78±1.07 U·mg protein-1 and 5.79±0.85 U·mg protein-1 in the alcoholic rats after 6 and 12 weeks, respectively. The LDH activity decreased to a lesser extent, but significantly: 2.45±0.18 U·mg protein-1 in the controls, and 2.11±0.07 U·mg protein-1 and 2.06±0.29 U·mg protein-1 after 6 and 12 weeks on test. Only slight, not significant, changes were observed for the other enzymes investigated (ICDH, ALD, GPT). The distribution of LDH and MDH isoenzymes, as well as changes in the MDH/LDH and MDH/ALD ratios, suggests a slowing-down of the oxidative processes and a relative preponderance of glycolysis in the alcoholic rats. The possible pathogenesis of the enzyme activity changes brought about by chronic alcohol ingestion is discussed.

Original languageEnglish
Pages (from-to)691-695
Number of pages5
JournalCardiovascular Research
Volume17
Issue number11
DOIs
Publication statusPublished - Jan 1 1983

Fingerprint

Eating
Alcohols
Malate Dehydrogenase
Enzymes
L-Lactate Dehydrogenase
Fructose-Bisphosphate Aldolase
Proteins
Creatine Kinase
Transaminases
Pyruvic Acid
Glutamic Acid
Myocardium
Isocitrate Dehydrogenase
Glycolysis
Isoenzymes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Enzyme activity changes in rat heart after chronic alcohol ingestion. / Édes, I.; Andó, Ágota; Csanády, Miklós; Mazareán, Hortenzia; Guba, Ferenc.

In: Cardiovascular Research, Vol. 17, No. 11, 01.01.1983, p. 691-695.

Research output: Contribution to journalReview article

Édes, I, Andó, Á, Csanády, M, Mazareán, H & Guba, F 1983, 'Enzyme activity changes in rat heart after chronic alcohol ingestion', Cardiovascular Research, vol. 17, no. 11, pp. 691-695. https://doi.org/10.1093/cvr/17.11.691
Édes, I. ; Andó, Ágota ; Csanády, Miklós ; Mazareán, Hortenzia ; Guba, Ferenc. / Enzyme activity changes in rat heart after chronic alcohol ingestion. In: Cardiovascular Research. 1983 ; Vol. 17, No. 11. pp. 691-695.
@article{6c0a2970680c45c79fc2487a43ddbef9,
title = "Enzyme activity changes in rat heart after chronic alcohol ingestion",
abstract = "Summary: The effect of a chronic intake of dietary alcohol upon myocardial enzymes was studied in rats. Alcohol, comprising more than 40{\%} of the dietary calorie content, was administered to rats for 6 or 12 weeks. To assess the metabolic changes in the myocardium, the following enzymes were measured: lactate dehydrogenase (LDH), malate dehydrogenase (MDH), aldolase (ALD), isocitrate dehydrogenase (ICDH), creatine kinase (CK) and glutamate-pyruvate transaminase (GPT).The activity of CK was decreased (4.79±0.99 U·mg-1 protein) after 6 weeks on alcohol and was significantly different from that of the controls (5.98±1.44 U·mg-1 protein). After 12 weeks the CK activity of alcoholic rats had recovered to 5.99±1.08 U·mg protein-1 and approached the value found in the normal myocardium. A pronounced decrease was found in the activity of MDH: 8.26±0.69 U·mg protein-1 in the controls, and 6.78±1.07 U·mg protein-1 and 5.79±0.85 U·mg protein-1 in the alcoholic rats after 6 and 12 weeks, respectively. The LDH activity decreased to a lesser extent, but significantly: 2.45±0.18 U·mg protein-1 in the controls, and 2.11±0.07 U·mg protein-1 and 2.06±0.29 U·mg protein-1 after 6 and 12 weeks on test. Only slight, not significant, changes were observed for the other enzymes investigated (ICDH, ALD, GPT). The distribution of LDH and MDH isoenzymes, as well as changes in the MDH/LDH and MDH/ALD ratios, suggests a slowing-down of the oxidative processes and a relative preponderance of glycolysis in the alcoholic rats. The possible pathogenesis of the enzyme activity changes brought about by chronic alcohol ingestion is discussed.",
author = "I. {\'E}des and {\'A}gota And{\'o} and Mikl{\'o}s Csan{\'a}dy and Hortenzia Mazare{\'a}n and Ferenc Guba",
year = "1983",
month = "1",
day = "1",
doi = "10.1093/cvr/17.11.691",
language = "English",
volume = "17",
pages = "691--695",
journal = "Cardiovascular Research",
issn = "0008-6363",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Enzyme activity changes in rat heart after chronic alcohol ingestion

AU - Édes, I.

AU - Andó, Ágota

AU - Csanády, Miklós

AU - Mazareán, Hortenzia

AU - Guba, Ferenc

PY - 1983/1/1

Y1 - 1983/1/1

N2 - Summary: The effect of a chronic intake of dietary alcohol upon myocardial enzymes was studied in rats. Alcohol, comprising more than 40% of the dietary calorie content, was administered to rats for 6 or 12 weeks. To assess the metabolic changes in the myocardium, the following enzymes were measured: lactate dehydrogenase (LDH), malate dehydrogenase (MDH), aldolase (ALD), isocitrate dehydrogenase (ICDH), creatine kinase (CK) and glutamate-pyruvate transaminase (GPT).The activity of CK was decreased (4.79±0.99 U·mg-1 protein) after 6 weeks on alcohol and was significantly different from that of the controls (5.98±1.44 U·mg-1 protein). After 12 weeks the CK activity of alcoholic rats had recovered to 5.99±1.08 U·mg protein-1 and approached the value found in the normal myocardium. A pronounced decrease was found in the activity of MDH: 8.26±0.69 U·mg protein-1 in the controls, and 6.78±1.07 U·mg protein-1 and 5.79±0.85 U·mg protein-1 in the alcoholic rats after 6 and 12 weeks, respectively. The LDH activity decreased to a lesser extent, but significantly: 2.45±0.18 U·mg protein-1 in the controls, and 2.11±0.07 U·mg protein-1 and 2.06±0.29 U·mg protein-1 after 6 and 12 weeks on test. Only slight, not significant, changes were observed for the other enzymes investigated (ICDH, ALD, GPT). The distribution of LDH and MDH isoenzymes, as well as changes in the MDH/LDH and MDH/ALD ratios, suggests a slowing-down of the oxidative processes and a relative preponderance of glycolysis in the alcoholic rats. The possible pathogenesis of the enzyme activity changes brought about by chronic alcohol ingestion is discussed.

AB - Summary: The effect of a chronic intake of dietary alcohol upon myocardial enzymes was studied in rats. Alcohol, comprising more than 40% of the dietary calorie content, was administered to rats for 6 or 12 weeks. To assess the metabolic changes in the myocardium, the following enzymes were measured: lactate dehydrogenase (LDH), malate dehydrogenase (MDH), aldolase (ALD), isocitrate dehydrogenase (ICDH), creatine kinase (CK) and glutamate-pyruvate transaminase (GPT).The activity of CK was decreased (4.79±0.99 U·mg-1 protein) after 6 weeks on alcohol and was significantly different from that of the controls (5.98±1.44 U·mg-1 protein). After 12 weeks the CK activity of alcoholic rats had recovered to 5.99±1.08 U·mg protein-1 and approached the value found in the normal myocardium. A pronounced decrease was found in the activity of MDH: 8.26±0.69 U·mg protein-1 in the controls, and 6.78±1.07 U·mg protein-1 and 5.79±0.85 U·mg protein-1 in the alcoholic rats after 6 and 12 weeks, respectively. The LDH activity decreased to a lesser extent, but significantly: 2.45±0.18 U·mg protein-1 in the controls, and 2.11±0.07 U·mg protein-1 and 2.06±0.29 U·mg protein-1 after 6 and 12 weeks on test. Only slight, not significant, changes were observed for the other enzymes investigated (ICDH, ALD, GPT). The distribution of LDH and MDH isoenzymes, as well as changes in the MDH/LDH and MDH/ALD ratios, suggests a slowing-down of the oxidative processes and a relative preponderance of glycolysis in the alcoholic rats. The possible pathogenesis of the enzyme activity changes brought about by chronic alcohol ingestion is discussed.

UR - http://www.scopus.com/inward/record.url?scp=85047676583&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047676583&partnerID=8YFLogxK

U2 - 10.1093/cvr/17.11.691

DO - 10.1093/cvr/17.11.691

M3 - Review article

VL - 17

SP - 691

EP - 695

JO - Cardiovascular Research

JF - Cardiovascular Research

SN - 0008-6363

IS - 11

ER -