Enzymatic synthesis of polyuridylic acid containing modified bases

Yau Kwan Ho, J. Aradi, Thomas J. Bardos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

5-Mercaptouridine-5′-diphosphate (hs5UDP) has been synthesized and investigated as a substrate of the polynucleotide phosphorylase of Micrococcusluteus. While hs5UDP is not utilized alone, It can be copolymerized with UDP; however, unusually for this enzyme, the ratio of 5-mercaptouridylate vs. uridylate residues in the polynucleotlde product (MPU) is always lower than the ratio of hs5UDP vs. UDP in the substrate mixture. Furthermore, hs5UDP decreases the rate of the enzymic polymerization reaction. The MPU productforms two-stranded and three-stranded complexes with poly(A). The circular dichroic spectra of these complexes are similar to those formed between poly(U) and poly(A), but their melting profiles indicate somewhat lower stability. The physicochemical and biochemical properties of the enzymic product are qualitatively similar to those of MPU prepared by chemical modification; both are potent inhibitors of a DNA-dependent RNA polyrmerase.

Original languageEnglish
Pages (from-to)3175-3192
Number of pages18
JournalNucleic Acids Research
Volume8
Issue number14
DOIs
Publication statusPublished - Jul 25 1980

Fingerprint

Poly U
Poly A
Uridine Diphosphate
Polyribonucleotide Nucleotidyltransferase
Substrate
Synthesis
Acids
Chemical modification
Polymerization
Substrates
RNA
Melting
Freezing
Inhibitor
Enzymes
DNA
Decrease
Dependent
5-mercaptouridine 5'-diphosphate
Profile

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Enzymatic synthesis of polyuridylic acid containing modified bases. / Ho, Yau Kwan; Aradi, J.; Bardos, Thomas J.

In: Nucleic Acids Research, Vol. 8, No. 14, 25.07.1980, p. 3175-3192.

Research output: Contribution to journalArticle

Ho, Yau Kwan ; Aradi, J. ; Bardos, Thomas J. / Enzymatic synthesis of polyuridylic acid containing modified bases. In: Nucleic Acids Research. 1980 ; Vol. 8, No. 14. pp. 3175-3192.
@article{fd8db4f0600e450db2019cfb5028705f,
title = "Enzymatic synthesis of polyuridylic acid containing modified bases",
abstract = "5-Mercaptouridine-5′-diphosphate (hs5UDP) has been synthesized and investigated as a substrate of the polynucleotide phosphorylase of Micrococcusluteus. While hs5UDP is not utilized alone, It can be copolymerized with UDP; however, unusually for this enzyme, the ratio of 5-mercaptouridylate vs. uridylate residues in the polynucleotlde product (MPU) is always lower than the ratio of hs5UDP vs. UDP in the substrate mixture. Furthermore, hs5UDP decreases the rate of the enzymic polymerization reaction. The MPU productforms two-stranded and three-stranded complexes with poly(A). The circular dichroic spectra of these complexes are similar to those formed between poly(U) and poly(A), but their melting profiles indicate somewhat lower stability. The physicochemical and biochemical properties of the enzymic product are qualitatively similar to those of MPU prepared by chemical modification; both are potent inhibitors of a DNA-dependent RNA polyrmerase.",
author = "Ho, {Yau Kwan} and J. Aradi and Bardos, {Thomas J.}",
year = "1980",
month = "7",
day = "25",
doi = "10.1093/nar/8.14.3175",
language = "English",
volume = "8",
pages = "3175--3192",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "14",

}

TY - JOUR

T1 - Enzymatic synthesis of polyuridylic acid containing modified bases

AU - Ho, Yau Kwan

AU - Aradi, J.

AU - Bardos, Thomas J.

PY - 1980/7/25

Y1 - 1980/7/25

N2 - 5-Mercaptouridine-5′-diphosphate (hs5UDP) has been synthesized and investigated as a substrate of the polynucleotide phosphorylase of Micrococcusluteus. While hs5UDP is not utilized alone, It can be copolymerized with UDP; however, unusually for this enzyme, the ratio of 5-mercaptouridylate vs. uridylate residues in the polynucleotlde product (MPU) is always lower than the ratio of hs5UDP vs. UDP in the substrate mixture. Furthermore, hs5UDP decreases the rate of the enzymic polymerization reaction. The MPU productforms two-stranded and three-stranded complexes with poly(A). The circular dichroic spectra of these complexes are similar to those formed between poly(U) and poly(A), but their melting profiles indicate somewhat lower stability. The physicochemical and biochemical properties of the enzymic product are qualitatively similar to those of MPU prepared by chemical modification; both are potent inhibitors of a DNA-dependent RNA polyrmerase.

AB - 5-Mercaptouridine-5′-diphosphate (hs5UDP) has been synthesized and investigated as a substrate of the polynucleotide phosphorylase of Micrococcusluteus. While hs5UDP is not utilized alone, It can be copolymerized with UDP; however, unusually for this enzyme, the ratio of 5-mercaptouridylate vs. uridylate residues in the polynucleotlde product (MPU) is always lower than the ratio of hs5UDP vs. UDP in the substrate mixture. Furthermore, hs5UDP decreases the rate of the enzymic polymerization reaction. The MPU productforms two-stranded and three-stranded complexes with poly(A). The circular dichroic spectra of these complexes are similar to those formed between poly(U) and poly(A), but their melting profiles indicate somewhat lower stability. The physicochemical and biochemical properties of the enzymic product are qualitatively similar to those of MPU prepared by chemical modification; both are potent inhibitors of a DNA-dependent RNA polyrmerase.

UR - http://www.scopus.com/inward/record.url?scp=0019332778&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019332778&partnerID=8YFLogxK

U2 - 10.1093/nar/8.14.3175

DO - 10.1093/nar/8.14.3175

M3 - Article

C2 - 7443515

AN - SCOPUS:0019332778

VL - 8

SP - 3175

EP - 3192

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 14

ER -