Enzymatic hydrolysis and biological activity of oligonucleotides containing 5-substituted pyrimidine bases

L. Ötvös, J. Sági, Gy Sági, A. Szemzõ, F. D. Tóth, A. Jeney

Research output: Contribution to journalArticle

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Two series of alternating ODNs containing 5-n alkyl-, alkenyl- and alkynyl-dU and -dC units have been prepared in order to study the kinetics of their hydrolysis by SV PDE and human serum, respectively. Both in (r5dUpdA)10 and (r5dCpdG)6 series the rate of hydrolysis decreased with increasing length of side-chain. Replacement of thymidines by 5-hexynyl-dU in different antisense oligomers resulted in considerably higher biological activity relative to that of the thymidine-containing counterparts.

Original languageEnglish
Pages (from-to)1665-1666
Number of pages2
JournalNucleosides and Nucleotides
Issue number6-7
Publication statusPublished - Jan 1 1999


ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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