Entrapment of high-molecular-mass DNA molecules in liposomes for the genetic transformation of animal cells

J. Szelei, E. Duda

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We modified the Ca/EDTA procedure for the production of liposomes [Papahadjopoulos, Vail, Jacobson & Poste (1975) Biochim. Biophys. Acta 394, 483-491] to entrap intact DNA molecules of very high molecular mass into large unilamellar phospholipid vesicles. The use of DNA-protein complexes and phage particles instead of naked linear DNA increases the efficiency of entrapment and protects the integrity of DNA molecules. We investigated the interaction of mammalian cells with liposome-encapsulated recombinant λ bacteriophages carrying marker genes. The liposomes bind surprisingly fast to the cellular surface and are taken up by the cells. A significant proportion of the encapsulated DNA is transported to and soon located in or around the nuclei. Experiments prove that these liposomes can be used for the genetic transformation of mammalian cells.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalBiochemical Journal
Volume259
Issue number2
Publication statusPublished - 1989

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Genetic Transformation
Molecular mass
Liposomes
Animals
Cells
Molecules
DNA
Bacteriophages
Unilamellar Liposomes
Edetic Acid
Cell Communication
Phospholipids
Genes
Proteins
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Entrapment of high-molecular-mass DNA molecules in liposomes for the genetic transformation of animal cells. / Szelei, J.; Duda, E.

In: Biochemical Journal, Vol. 259, No. 2, 1989, p. 549-553.

Research output: Contribution to journalArticle

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