Fecal samples were collected from sheltered dogs in the north-eastern region of Hungary during 2012. Individual PCR and RT-PCR assays were performed to detect the presence of major viral enteric pathogens, as well as less known viruses. The methods were used to detect canine parvovirus, coronavirus, calicivirus, kobuvirus, astrovirus and rotavirus. To confirm the PCR results the amplified genomic regions were sequenced and the obtained data were used for phylogenetic analysis. The prevalence of different viruses showed some variations (e.g., calicivirus, 4%; coronavirus, 33%). Co-infection with multiple viruses was detected in 25% of the samples. Viral shedding was very common in asymptomatic animals. The analysis of the genetic diversity of enteric viruses confirmed the presence of a novel astrovirus and potential new variants of known canine enteric pathogens, such as corona-, noro-, and vesiviruses. To the best of the authors' knowledge, they are the first to report the occurrence of astro-, kobu-, rota-, and calicivirus in dogs in Hungary.
|Translated title of the contribution||Enteric viral infections of sheltered dogs in Hungary|
|Number of pages||10|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Nov 1 2014|
ASJC Scopus subject areas