Enhancing the quality of separation in one-dimensional peptide mapping using mathematical transformation

Róbert Berky, Szabolcs Fekete, J. Fekete

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this study, some practical examples are presented that show the quality of separations using very efficient columns packed with the latest generation of core shell sub-3 lm and fully porous sub-2 lm particles in onedimensional peptide separations. This paper shows an approach for the analysis of proteins, such as high-resolution separations, and a data transformation process to improve peak recognition and analysis. Applying power functions on raw chromatographic data can be a neat tool in the field of biosimilar analysis, especially in comparability studies regarding the quality (primary structure) of proteins. Based on the results presented here, it can be stated that the use of power functions is beneficial for the comparison of chromatograms when peak areas are considered but has no effect when using peak heights. In this study, the new Acquity CSH columns (C18 and phenyl-hexyl) and the core-shell type wide pore Ascentis Express Peptide ES C18 material were applied with great success in peptide mapping. Finally, using phenyl-hexyl stationary phase in peptide separation seems to be a good alternative to the generally applied C18 or C4 phases.

Original languageEnglish
Pages (from-to)305-312
Number of pages8
JournalChromatographia
Volume75
Issue number5-6
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Peptide Mapping
Mathematical transformations
Biosimilar Pharmaceuticals
Peptides
Amino Acid Sequence
Proteins

Keywords

  • Acquity CSH column
  • Biosimilar
  • Core-shell particles
  • Enhanced resolution
  • Liquid chromatography
  • Peptide map

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Enhancing the quality of separation in one-dimensional peptide mapping using mathematical transformation. / Berky, Róbert; Fekete, Szabolcs; Fekete, J.

In: Chromatographia, Vol. 75, No. 5-6, 03.2012, p. 305-312.

Research output: Contribution to journalArticle

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