Enhancer substances: Selegiline and R-(-)-1-(benzofuran-2-yl)-2-propylaminopentane [(-)-BPAP] enhance the neurotrophic factor synthesis on cultured mouse astrocytes

Seiichiro Shimazu, Akie Tanigawa, Noriyuki Sato, Fumio Yoneda, Kyozo Hayashi, Joseph Knoll

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

R-(-)-1-(Benzofuran-2-yl)-2-propylaminopentane [(-)-BPAP] is a potent "catecholaminergic and serotonergic activity enhancer (CAE/SAE)", which enhances the impulse-evoked catecholamines and serotonin release, e.g. (-)-BPAP enhances in vitro norepinephrine efflux from the slices of locus coeruleus in a bipolar manner with the two effective ranges of low (fM-pM level) and high (nM-μM level) concentrations. Here, the effects of (-)-BPAP and selegiline on the cultured mouse astrocytes were studied. The protein levels of the neurotrophic factors (NGF, BDNF and GDNF) in the conditioned medium of cultured astrocytes were determined by using ELISA. In the cultured astrocytes incubated for 24 h with selegiline, the synthesis of NGF and BDNF was significantly enhanced in the concentration dependent manner, with minimum effective concentrations of 4 × 10-4 and 5 × 10-4 M, respectively. (-)-BPAP also enhanced the NGF, BDNF and GDNF synthesis, with minimum effective concentrations of 5 × 10-5, 1 × 10-5, and 1 × 10-6 M, respectively. Although the effects of (-)-BPAP on the NGF synthesis was tested in the range of 1 × 10-15-5 × 10-4 M, the concentration response curve of (-)-BPAP was a single bell shape with the peak effect at 1 × 10-4 M, and did not show any effects in low concentrations such as fM-pM level. Each concentration response curve of (-)-BPAP on BDNF and GDNF synthesis was a single bell shape with peak effects at 1 × 10-3 M and 1 × 10-4 M, respectively.

Original languageEnglish
Pages (from-to)2785-2792
Number of pages8
JournalLife sciences
Volume72
Issue number24
DOIs
Publication statusPublished - May 2 2003

Keywords

  • Apomorphine
  • BDNF
  • Cultured mouse astrocytes
  • GDNF
  • NGF
  • Parkinson's disease
  • R-(-)-1-(Benzofuran-2-yl)-2-propylaminopentane [(-)-BPAP]
  • Selegiline [(-)-Deprenyl]

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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