Enhancement of the thermostability of Candida cylindracea lipase by medium engineering

L. Gubicza, K. Székely, O. Ulbert, K. Bélafi-Bakó

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The possibility to increase the thermostability of the enzyme Candida cylindracea lipase was studied using the medium engineering method. The purpose was to shift the property of the enzyme obtained from mesophilic organism towards thermophilic character and hence widen its application possibilities. Applying the heptane solvent as reaction medium, the relation between the water content of the reaction mixture and the enzyme activity was investigated in the temperature range of 30°C and 50°C. It was found that decreasing the water content of the reaction mixture, the activity of the enzyme was reduced, while its thermostability increased. The slower deactivation of the enzyme was explained by the reduced amount of water present, necessary for the denaturation reactions.

Original languageEnglish
Pages (from-to)351-354
Number of pages4
JournalChemical Papers
Volume54
Issue number6
Publication statusPublished - Dec 1 2000

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Materials Chemistry

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