Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L.

Péter Bányai, Viktor József Vojnich, Ákos Máthé, László Kursinszki, Éva Szőke

Research output: Contribution to journalArticle


Lobelia inflata L. is a medicinally important species that contains numerous piperidine alkaloids. The main alkaloid lobeline is used as a respiratory stimulant. Recently, there have been studies that report on its effect on the central nervous system, drug abuse, and multidrug resistance. We have studied the piperidine alkaloid formation of in vitro cultivated L. inflata on solid Murashige and Skoog medium. With the aim of increasing the anti-addictive lobeline production, we have investigated the effect of changing MgSO4 levels of the medium. The compounds (−)-lobeline, norlobelanine, lobelanidine-(−)-8-ethyl-10-phenyl-norlobelionol, (−)-8-ethyl-10-phenyl-lobelionol, norlobeline, lobelidine, and lobelanine were identified by HPLC-tandem mass spectrometry (MS/MS). We studied the effect of changing the MgSO4 levels on the content of lobeline and its derivatives for in vitro cultivated L. inflata. The highest lobeline content was measured in roots cultured on 185 mg L−1 MgSO4-containing medium; this value was 2.5 times higher (232.4 μg g−1) than in the control cultures. The greatest (fourfold) increase was observed in lobelidine (71.61 μg g−1). Addition of MgSO4 at 740 mg L−1 resulted in the highest lobeline contents in the herba (aerial tissues; 267.8 μg g−1), whereas maximum lobeline production was achieved by a further increase in the MgSO4 level (1,480 mg L−1), due to an intensive increase in biomass production. A slight increase was observed in the amount of lobeline derivatives in the herba grown in 740 mg L−1 MgSO4-containing medium. The most significant increase (25%) was recorded in the level of norlobelanine.

Original languageEnglish
Pages (from-to)760-765
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Plant
Issue number6
Publication statusPublished - Dec 19 2014


  • Lobelia inflata
  • Lobeline
  • Lobeline derivatives
  • MgSO
  • Micropropagation

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

Fingerprint Dive into the research topics of 'Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L.'. Together they form a unique fingerprint.

  • Cite this