Enhancement of chicken primordial germ cell in vitro maintenance using an automated cell image analyser

Mahek Anand, Bence Lázár, Roland Tóth, Emőke Páll, Eszter Patakiné Várkonyi, Krisztina Liptói, László Homolya, Zoltán Hegyi, András Hidas, Elen Gócza

Research output: Contribution to journalArticle

Abstract

Primordial germ cells (PGCs) were isolated from blood samples of chicken embryos. We established four PGC lines: two males (FS-ZZ-101, GFP-ZZ-4ZP) and two females (FS-ZW-111, GFP-ZW-5ZP). We could not detect a significant difference in the marker expression profile, but there was a remarkable difference between the proliferation rates of these PGC lines. We monitored the number of PGCs throughout a three-day period using a high-content screening cell imaging and analysing system (HCS). We compared three different initial cell concentrations in the wells: ~1000 cells (1×), ~4000 (4×) and ~8000 (8×). For the GFP-ZW-5ZP, FS-ZZ-101 and FS-ZW-111 PGC lines the lowest doubling time was observed at 4× concentration, while for GFP-ZZ-4ZP we found the lowest doubling time at 1× concentration. At 8× initial concentration, the growth rate was high during the first two days for all cell lines, but this was followed by the appearance of cell aggregates decreasing the cell growth rate. We could conclude that the difference in proliferation rate could mainly be attributed to genotypic variation in the established PGC lines, but external factors such as cell concentration and quality of the culture medium also affect the growth rate of PGCs.

Original languageEnglish
Pages (from-to)518-529
Number of pages12
JournalActa veterinaria Hungarica
Volume66
Issue number4
DOIs
Publication statusPublished - Dec 2018

Keywords

  • Cell culture
  • Chicken
  • Doubling time
  • High-content screening
  • Primordial germ cells
  • Proliferation rate

ASJC Scopus subject areas

  • veterinary(all)

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    Anand, M., Lázár, B., Tóth, R., Páll, E., Patakiné Várkonyi, E., Liptói, K., Homolya, L., Hegyi, Z., Hidas, A., & Gócza, E. (2018). Enhancement of chicken primordial germ cell in vitro maintenance using an automated cell image analyser. Acta veterinaria Hungarica, 66(4), 518-529. https://doi.org/10.1556/004.2018.046