Enhanced stability of polyacrylate-coated magnetite nanoparticles in biorelevant media

Angéla Hajdú, M. Szekeres, Ildikó Y. Tóth, Rita A. Bauer, J. Mihály, I. Zupkó, E. Tombácz

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Magnetite nanoparticles (MNPs) were prepared by alkaline hydrolysis of Fe(II) and Fe(III) chlorides. Adsorption of polyacrylic acid (PAA) on MNPs was measured at pH = 6.5 ± 0.3 and I= 0.01. M (NaCl) to find the optimal PAA amount for MNP stabilization under physiological conditions. We detected an H-bond formation between magnetite surface groups and PAA by ATR-FTIR measurements, but bonds of metal ion-carboxylate complexes, generally cited in literature, were not identified at the given pH and ionic strength. The dependence of the electrokinetic potential and the aggregation state on the amount of added PAA at various pHs was measured by electrophoretic mobility and dynamic light-scattering methods. The electrokinetic potential of the naked MNPs was low at near physiological pH, but PAA adsorption overcharged the particles. Highly negatively charged, well-stabilized carboxylated MNPs formed via adsorption of PAA in an amount of approximately ten times of that necessary to compensate the original positive charge of the magnetite. Coagulation kinetics experiments revealed gradual enhancement of salt tolerance at physiological pH from ∼0.001. M at no added PAA up to ∼0.5. M at 1.12. mmol/g PAA. The PAA-coated MNPs exert no substantial effect on the proliferation of malignant (HeLa) or non-cancerous fibroblast cells (MRC-5) as determined by means of MTT assays.

Original languageEnglish
Pages (from-to)242-249
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume94
DOIs
Publication statusPublished - Jun 1 2012

Fingerprint

carbopol 940
Magnetite Nanoparticles
Magnetite nanoparticles
acrylic resins
Polyacrylates
magnetite
nanoparticles
acids
Acids
Ferrosoferric Oxide
Adsorption
electrokinetics
Magnetite
adsorption
Salt-Tolerance
Electrophoretic mobility
fibroblasts
Dynamic light scattering
Fourier Transform Infrared Spectroscopy
Fibroblasts

Keywords

  • Carboxylated coating
  • Electrosteric stabilization
  • Magnetic fluids
  • Magnetite
  • Nanotoxicity
  • Polyacrylic acid

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Enhanced stability of polyacrylate-coated magnetite nanoparticles in biorelevant media. / Hajdú, Angéla; Szekeres, M.; Tóth, Ildikó Y.; Bauer, Rita A.; Mihály, J.; Zupkó, I.; Tombácz, E.

In: Colloids and Surfaces B: Biointerfaces, Vol. 94, 01.06.2012, p. 242-249.

Research output: Contribution to journalArticle

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