Enhanced production of industrial enzymes in Mucoromycotina fungi during solid-state fermentation of agricultural wastes/by-products

Miklós Takó, Alexandra Kotogán, Judit Krisch, C. Vágvölgyi, Keshab C. Mondal, T. Papp

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cellulolytic, lipolytic and proteolytic enzyme production of zygomycetes Mucor corticolus, Rhizomucor miehei, Gilbertella persicaria and Rhizopus niveus were investigated using agro-industrial wastes as substrates. Solid-state cultures were carried out on untreated corn residues (stalk and leaf) as single substrate (SSF1) or corn residues and wheat bran in mixed fermentation (SSF2). Rapid production of endoglucanase (CMCase) was observed with maximal activity reaching after about 48-h fermentation, while cellobiohydrolase (CBH) and β-glucosidase enzymes generally had their peak after 72-h incubation. Highest filter paper degrading (FPase), CMCase, CBH and β-glucosidase activities obtained were (U g-1 dss) 17.3, 74.1, 12.2 and 158.3, for R. miehei, G. persicaria, M. corticolus and Rh. niveus, respectively. M. corticolus proved to be the best lipolytic enzyme producer in SSF1 presenting 447.6 U g-1 dss yield, while R. miehei showed 517.7 U g-1 dss activity in SSF2. Rh. niveus exhibited significantly greater protease production than the other strains. Suc-AAPF-pNA hydrolyzing activities of this strain were 1.1 and 1.96 U g-1 dss in SSF1 and SSF2, respectively. We conclude that the used corn stalk and leaf residues could potentially be applicable as strong inducers for cellulase and lipase production by Mucoromycotina fungi.

Original languageEnglish
Pages (from-to)348-360
Number of pages13
JournalActa Biologica Hungarica
Volume66
Issue number3
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Waste Products
Agricultural wastes
Fungi
Cellulose 1,4-beta-Cellobiosidase
Fermentation
Glucosidases
Zea mays
fermentation
Byproducts
Cellulase
maize
fungus
enzyme
Peptide Hydrolases
Enzymes
Rhizomucor
Industrial Waste
Mucor
substrate
Rhizopus

Keywords

  • Corn stalk and leaf
  • Hydrolytic enzymes
  • Mixed fermentation
  • Wheat bran
  • Zygomycetes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology
  • Medicine(all)

Cite this

Enhanced production of industrial enzymes in Mucoromycotina fungi during solid-state fermentation of agricultural wastes/by-products. / Takó, Miklós; Kotogán, Alexandra; Krisch, Judit; Vágvölgyi, C.; Mondal, Keshab C.; Papp, T.

In: Acta Biologica Hungarica, Vol. 66, No. 3, 01.09.2015, p. 348-360.

Research output: Contribution to journalArticle

@article{31f0da0c612e4f548269a8c5cf670c2f,
title = "Enhanced production of industrial enzymes in Mucoromycotina fungi during solid-state fermentation of agricultural wastes/by-products",
abstract = "Cellulolytic, lipolytic and proteolytic enzyme production of zygomycetes Mucor corticolus, Rhizomucor miehei, Gilbertella persicaria and Rhizopus niveus were investigated using agro-industrial wastes as substrates. Solid-state cultures were carried out on untreated corn residues (stalk and leaf) as single substrate (SSF1) or corn residues and wheat bran in mixed fermentation (SSF2). Rapid production of endoglucanase (CMCase) was observed with maximal activity reaching after about 48-h fermentation, while cellobiohydrolase (CBH) and β-glucosidase enzymes generally had their peak after 72-h incubation. Highest filter paper degrading (FPase), CMCase, CBH and β-glucosidase activities obtained were (U g-1 dss) 17.3, 74.1, 12.2 and 158.3, for R. miehei, G. persicaria, M. corticolus and Rh. niveus, respectively. M. corticolus proved to be the best lipolytic enzyme producer in SSF1 presenting 447.6 U g-1 dss yield, while R. miehei showed 517.7 U g-1 dss activity in SSF2. Rh. niveus exhibited significantly greater protease production than the other strains. Suc-AAPF-pNA hydrolyzing activities of this strain were 1.1 and 1.96 U g-1 dss in SSF1 and SSF2, respectively. We conclude that the used corn stalk and leaf residues could potentially be applicable as strong inducers for cellulase and lipase production by Mucoromycotina fungi.",
keywords = "Corn stalk and leaf, Hydrolytic enzymes, Mixed fermentation, Wheat bran, Zygomycetes",
author = "Mikl{\'o}s Tak{\'o} and Alexandra Kotog{\'a}n and Judit Krisch and C. V{\'a}gv{\"o}lgyi and Mondal, {Keshab C.} and T. Papp",
year = "2015",
month = "9",
day = "1",
doi = "10.1556/018.66.2015.3.10",
language = "English",
volume = "66",
pages = "348--360",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - Enhanced production of industrial enzymes in Mucoromycotina fungi during solid-state fermentation of agricultural wastes/by-products

AU - Takó, Miklós

AU - Kotogán, Alexandra

AU - Krisch, Judit

AU - Vágvölgyi, C.

AU - Mondal, Keshab C.

AU - Papp, T.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Cellulolytic, lipolytic and proteolytic enzyme production of zygomycetes Mucor corticolus, Rhizomucor miehei, Gilbertella persicaria and Rhizopus niveus were investigated using agro-industrial wastes as substrates. Solid-state cultures were carried out on untreated corn residues (stalk and leaf) as single substrate (SSF1) or corn residues and wheat bran in mixed fermentation (SSF2). Rapid production of endoglucanase (CMCase) was observed with maximal activity reaching after about 48-h fermentation, while cellobiohydrolase (CBH) and β-glucosidase enzymes generally had their peak after 72-h incubation. Highest filter paper degrading (FPase), CMCase, CBH and β-glucosidase activities obtained were (U g-1 dss) 17.3, 74.1, 12.2 and 158.3, for R. miehei, G. persicaria, M. corticolus and Rh. niveus, respectively. M. corticolus proved to be the best lipolytic enzyme producer in SSF1 presenting 447.6 U g-1 dss yield, while R. miehei showed 517.7 U g-1 dss activity in SSF2. Rh. niveus exhibited significantly greater protease production than the other strains. Suc-AAPF-pNA hydrolyzing activities of this strain were 1.1 and 1.96 U g-1 dss in SSF1 and SSF2, respectively. We conclude that the used corn stalk and leaf residues could potentially be applicable as strong inducers for cellulase and lipase production by Mucoromycotina fungi.

AB - Cellulolytic, lipolytic and proteolytic enzyme production of zygomycetes Mucor corticolus, Rhizomucor miehei, Gilbertella persicaria and Rhizopus niveus were investigated using agro-industrial wastes as substrates. Solid-state cultures were carried out on untreated corn residues (stalk and leaf) as single substrate (SSF1) or corn residues and wheat bran in mixed fermentation (SSF2). Rapid production of endoglucanase (CMCase) was observed with maximal activity reaching after about 48-h fermentation, while cellobiohydrolase (CBH) and β-glucosidase enzymes generally had their peak after 72-h incubation. Highest filter paper degrading (FPase), CMCase, CBH and β-glucosidase activities obtained were (U g-1 dss) 17.3, 74.1, 12.2 and 158.3, for R. miehei, G. persicaria, M. corticolus and Rh. niveus, respectively. M. corticolus proved to be the best lipolytic enzyme producer in SSF1 presenting 447.6 U g-1 dss yield, while R. miehei showed 517.7 U g-1 dss activity in SSF2. Rh. niveus exhibited significantly greater protease production than the other strains. Suc-AAPF-pNA hydrolyzing activities of this strain were 1.1 and 1.96 U g-1 dss in SSF1 and SSF2, respectively. We conclude that the used corn stalk and leaf residues could potentially be applicable as strong inducers for cellulase and lipase production by Mucoromycotina fungi.

KW - Corn stalk and leaf

KW - Hydrolytic enzymes

KW - Mixed fermentation

KW - Wheat bran

KW - Zygomycetes

UR - http://www.scopus.com/inward/record.url?scp=84946415836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946415836&partnerID=8YFLogxK

U2 - 10.1556/018.66.2015.3.10

DO - 10.1556/018.66.2015.3.10

M3 - Article

VL - 66

SP - 348

EP - 360

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 3

ER -