Enhanced glutathione metabolism is correlated with sulfur-induced resistance in tobacco mosaic virus-infected genetically susceptible nicotiana tabacum plants

Kerstin Höller, Lóránt Király, András Künstler, Maria Müller, G. Gullner, Maria Fattinger, Bernd Zechmann

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Sulfur-induced resistance, also known as sulfur-enhanced defense (SIR/SED) was investigated in Nicotiana tabacum cv. Samsun nn during compatible interaction with Tobacco mosaic virus (TMV) in correlation with glutathione metabolism. To evaluate the influence of sulfur nutritional status on virus infection, tobacco plants were treated with nutrient solutions containing either sufficient sulfate (+S) or no sulfate (-S). Sufficient sulfate supply resulted in a suppressed and delayed symptom development and diminished virus accumulation over a period of 14 days after inoculation as compared with -S conditions. Expression of the defense marker gene PR-1a was markedly upregulated in sulfate-treated plants during the first day after TMV inoculation. The occurrence of SIR/SED correlated with a higher level of activity of sulfate assimilation, cysteine, and gluta- thione metabolism in plants treated with sulfate. Additionally, two key genes involved in cysteine and glutathione biosynthesis (encoding adenosine 5'-phosphosulfate reductase and y-glutamylcysteine synthetase, respectively) were up- regulated within the first day after TMV inoculation under +S conditions. Sulfate withdrawal from the soil was accelerated at the beginning of the infection, whereas it declined in the long term, leading to an accumulation of sulfur in the soil of plants grown with sulfate. This observation could be correlated with a decrease in sulfur contents in TMV-infected leaves in the long term. In summary, this is the first study that demonstrates a link between the activation of cysteine and glutathione metabolism and the induction of SIR/SED during a compatible plant-virus interaction in tobacco plants, indicating a general mechanism behind SIR/SED.

Original languageEnglish
Pages (from-to)1448-1459
Number of pages12
JournalMolecular Plant-Microbe Interactions
Volume23
Issue number11
DOIs
Publication statusPublished - Nov 2010

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Tobacco Mosaic Virus
Tobacco mosaic virus
induced resistance
Sulfur
Nicotiana tabacum
Tobacco
Glutathione
glutathione
sulfur
Sulfates
sulfates
metabolism
Cysteine
cysteine
tobacco
Soil
adenylyl-sulfate reductase
Glutamate-Cysteine Ligase
Plant Viruses
viruses

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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Enhanced glutathione metabolism is correlated with sulfur-induced resistance in tobacco mosaic virus-infected genetically susceptible nicotiana tabacum plants. / Höller, Kerstin; Király, Lóránt; Künstler, András; Müller, Maria; Gullner, G.; Fattinger, Maria; Zechmann, Bernd.

In: Molecular Plant-Microbe Interactions, Vol. 23, No. 11, 11.2010, p. 1448-1459.

Research output: Contribution to journalArticle

Höller, Kerstin ; Király, Lóránt ; Künstler, András ; Müller, Maria ; Gullner, G. ; Fattinger, Maria ; Zechmann, Bernd. / Enhanced glutathione metabolism is correlated with sulfur-induced resistance in tobacco mosaic virus-infected genetically susceptible nicotiana tabacum plants. In: Molecular Plant-Microbe Interactions. 2010 ; Vol. 23, No. 11. pp. 1448-1459.
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