Enhanced expression of glucose-regulated protein 78 correlates with malondialdehyde levels during the formation of liver cirrhosis in rats

Yun Zhang, Huiying Zhang, Zhongfu Zhao, Minli Lv, Jiantao Jia, Lili Zhang, Xiaoxia Tian, Yunxia Chen, Baohong Li, Mingshe Liu, Dewu Han, Cheng Ji

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of the present study was to explore the role of glucose-regulated protein 78 (GRP78) in the development of liver cirrhosis promoted by intestinal endotoxemia in rats. Fifty-one male Wistar rats were randomly divided into the liver cirrhosis 4-week, 6-week and 8-week groups and the normal control group at each time point. Liver cirrhosis was induced by employing multiple pathogenic factors in the rats. Blood and liver tissues were collected. The levels of alanine aminotransferase (ALT), homocysteine, endotoxin and tumor necrosis factor-α (TNF-α) in the plasma, and TNF-α, malondialdehyde (MDA) and procollagen type III peptide (PIIIP) in the liver tissues were determined. The mRNA and protein expression levels of GRP78 in the liver were detected using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry. Morphological changes were observed through hematoxylin and eosin and van Gieson staining of the liver. Liver cirrhosis caused marked histopathological changes to the livers of the rats. Following significant increases in the levels of ALT, homocysteine, endotoxin and TNF-α in the plasma, and TNF-α, MDA and PIIIP in the liver tissues of all experimental groups with the progression of liver cirrhosis, the mRNA and protein expression levels of GRP78 also gradually increased. In addition, correlation analysis indicated that the enhanced expression of GRP78 correlated with the MDA levels of the rats during the formation of liver cirrhosis.

Original languageEnglish
Pages (from-to)2119-2125
Number of pages7
JournalExperimental and Therapeutic Medicine
Volume10
Issue number6
DOIs
Publication statusPublished - Dec 2015

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Keywords

  • Endoplasmic reticulum stress
  • Endotoxin
  • Glucose-regulated protein 78
  • Homocysteine
  • Intestinal endotoxemia
  • Liver cirrhosis

ASJC Scopus subject areas

  • Immunology and Microbiology (miscellaneous)
  • Cancer Research

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