Enhanced expression of CGRP in rat trigeminal ganglion neurons during cell and organ culture

Anikó Kuris, Cang Bao Xu, Ming Fang Zhou, J. Tajti, Rolf Uddman, Lars Edvinsson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The sensory innervation of intracranial vessels originates in the trigeminal ganglion with calcitonin gene-related peptide (CGRP), substance P (SP) and pituitary adenylate cyclase activating peptide (PACAP) as frequent neuronal messengers. The present study was designed to study the expression of these neuropeptides (a) in primary culture of adult rat trigeminal ganglion neuronal cells and (b) in organ culture of sections of the trigeminal ganglion. In cell culture, axons grow in the peripheral direction for up to 48 h. Immunocytochemistry revealed that the cell bodies showed increased expression of CGRP at 24 h and SP at 24-48 h (p <0.05), whereas cell culture did not increase the expression of PACAP at 24 h (p > 0.05), but at 48 h (p <0.05). A significant elevation of CGRP mRNA was seen at 12 h, i.e. before the increased CGRP immunoreaction was observed. In organ culture of sections of trigeminal ganglia, the number of CGRP immunoreactive (-ir) cells and the mRNA expression were significantly increased at 24 and 48 h of incubation as compared to control (p <0.05), while the number of SP-ir cells was not altered (p > 0.05). In conclusion, neurons of rat trigeminal ganglia alter their expression of neuropeptides during cell and organ culture differently, but it is mainly the CGRP system that is up-regulated. We have compared two methods for future studies of underlying molecular mechanisms responsible for regulation of neuropeptide expression in the trigeminal system.

Original languageEnglish
Pages (from-to)6-13
Number of pages8
JournalBrain Research
Volume1173
Issue number1
DOIs
Publication statusPublished - Oct 10 2007

Fingerprint

Trigeminal Ganglion
Calcitonin Gene-Related Peptide
Organ Culture Techniques
Cell Culture Techniques
Neuropeptides
Neurons
Substance P
Adenylyl Cyclases
Axons
Immunohistochemistry
Peptides

Keywords

  • Immunocytochemistry
  • Migraine
  • Neuropeptide
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Enhanced expression of CGRP in rat trigeminal ganglion neurons during cell and organ culture. / Kuris, Anikó; Xu, Cang Bao; Zhou, Ming Fang; Tajti, J.; Uddman, Rolf; Edvinsson, Lars.

In: Brain Research, Vol. 1173, No. 1, 10.10.2007, p. 6-13.

Research output: Contribution to journalArticle

Kuris, Anikó ; Xu, Cang Bao ; Zhou, Ming Fang ; Tajti, J. ; Uddman, Rolf ; Edvinsson, Lars. / Enhanced expression of CGRP in rat trigeminal ganglion neurons during cell and organ culture. In: Brain Research. 2007 ; Vol. 1173, No. 1. pp. 6-13.
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