Enhanced cardiac differentiation of mouse embryonic stem cells by use of the slow-turning, lateral vessel (STLV) bioreactor

Sasitorn Rungarunlert, Nuttha Klincumhom, Istvan Bock, Csilla Nemes, Mongkol Techakumphu, M. Pirity, A. Dinnyés

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Embryoid body (EB) formation is a common intermediate during in vitro differentiation of pluripotent stem cells into specialized cell types. We have optimized the slow-turning, lateral vessel (STLV) for large scale and homogenous EB production from mouse embryonic stem cells. The effects of inoculating different cell numbers, time of EB adherence to gelatin-coated dishes, and rotation speed for optimal EB formation and cardiac differentiation were investigated. Using 3 × 10 5 cells/ml, 10 rpm rotary speed and plating of EBs onto gelatin-coated surfaces three days after culture, were the best parameters for optimal size and EB quality on consequent cardiac differentiation. These optimized parameters enrich cardiac differentiation in ES cells when using the STLV method.

Original languageEnglish
Pages (from-to)1565-1573
Number of pages9
JournalBiotechnology Letters
Volume33
Issue number8
DOIs
Publication statusPublished - Aug 2011

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Embryoid Bodies
Bioreactors
Gelatin
Stem cells
Plating
Pluripotent Stem Cells
Cell Count
Mouse Embryonic Stem Cells

Keywords

  • Bioreactor
  • Cardiomyocyte
  • Embryoid body
  • Embryonic stem cells
  • Slow turning lateral vessel

ASJC Scopus subject areas

  • Biotechnology

Cite this

Enhanced cardiac differentiation of mouse embryonic stem cells by use of the slow-turning, lateral vessel (STLV) bioreactor. / Rungarunlert, Sasitorn; Klincumhom, Nuttha; Bock, Istvan; Nemes, Csilla; Techakumphu, Mongkol; Pirity, M.; Dinnyés, A.

In: Biotechnology Letters, Vol. 33, No. 8, 08.2011, p. 1565-1573.

Research output: Contribution to journalArticle

Rungarunlert, Sasitorn ; Klincumhom, Nuttha ; Bock, Istvan ; Nemes, Csilla ; Techakumphu, Mongkol ; Pirity, M. ; Dinnyés, A. / Enhanced cardiac differentiation of mouse embryonic stem cells by use of the slow-turning, lateral vessel (STLV) bioreactor. In: Biotechnology Letters. 2011 ; Vol. 33, No. 8. pp. 1565-1573.
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AU - Nemes, Csilla

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