Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains

Gopalakrishnan Kumar, Péter Bakonyi, Periyasamy Sivagurunathan, Sang Hyoun Kim, N. Nemestóthy, K. Bélafi-Bakó, Chiu Yue Lin

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In this work biohydrogen generation and its improvement possibilities from beverage industrial wastewater were sought. Firstly, mesophilic hydrogen fermentations were conducted in batch vials by applying heat-treated (80°C, 30 min) sludge and liquid (LB-grown) cultures of Escherichia coli XL1-Blue/. Enterobacter cloacae DSM 16657 strains for bioaugmentation purposes. The results showed that there was a remarkable increase in hydrogen production capacities when facultative anaerobes were added in the form of inoculum. Furthermore, experiments were carried out in order to reveal whether the increment occurred either due to the efficient contribution of the facultative anaerobic microorganisms or the culture ingredients (in particular yeast extract and tryptone) supplied when the bacterial suspensions (LB media-based inocula) were mixed with the sludge. The outcome of these tests was that both the applied nitrogen sources and the bacteria (. E. coli) could individually enhance hydrogen formation. Nevertheless, the highest increase took place when they were used together. Finally, the optimal initial wastewater concentration was determined as 5 g/L.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume120
Issue number2
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

Beverages
Waste Water
Escherichia coli
Hydrogen
Wastewater
Nitrogen
Sewage
Hydrogen production
Microorganisms
Yeast
Fermentation
Enterobacter cloacae
Suspensions
Bacteria
Liquids
Hot Temperature
Yeasts
Experiments
tryptones

Keywords

  • Beverage wastewater
  • Bioaugmentation
  • Enterobacter cloacae DSM 16657
  • Escherichia coli XL1-Blue
  • External nitrogen source

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains. / Kumar, Gopalakrishnan; Bakonyi, Péter; Sivagurunathan, Periyasamy; Kim, Sang Hyoun; Nemestóthy, N.; Bélafi-Bakó, K.; Lin, Chiu Yue.

In: Journal of Bioscience and Bioengineering, Vol. 120, No. 2, 01.08.2015, p. 155-160.

Research output: Contribution to journalArticle

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