Enhanced antibody-dependent cell-mediated cytotoxicity of peripheral blood mononuclear cells in patients with germinal cell tumors

R. Kirschner, C. Kulhavi, I. Bodrogi, M. Boki, P. Siklos, T. Bakacs

Research output: Contribution to journalArticle

Abstract

The antibody-dependent cell mediated cytotoxicity (K-cell activity) of peripheral blood mononuclear cells from 92 patients with germinal cell tumors and 60 healthy male controls was measured against 0,Rh(D) positive human red blood cells sensitized with anti-D antibody. To determine the maximal K-cell activity the enyzme-like kinetic model of cytotoxicity-in which lysis was measured in presence of target cell excess, was utilized. To avoid variations due to the individual sensitivity of target erythrocytes red blood cells were obtained from a single donor. It was demonstrated that the R-cell activity of patients with germinal cell tumors was significantly enhanced compared to the control group. Patients with clinically detectable tumors showed relevant higher activity than those without detectable tumor. Chemotherapy significantly enhanced the activity of patients in the former group.

Original languageEnglish
Pages (from-to)61-65
Number of pages5
JournalJournal of Experimental and Clinical Cancer Research
Volume13
Issue number1
Publication statusPublished - 1994

Fingerprint

Antibody-Dependent Cell Cytotoxicity
Blood Cells
Neoplasms
Erythrocytes
Anti-Idiotypic Antibodies
Tissue Donors
Drug Therapy
Control Groups

Keywords

  • ADCC
  • chemotherapy
  • germinal cell tumor
  • K-cell

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Enhanced antibody-dependent cell-mediated cytotoxicity of peripheral blood mononuclear cells in patients with germinal cell tumors. / Kirschner, R.; Kulhavi, C.; Bodrogi, I.; Boki, M.; Siklos, P.; Bakacs, T.

In: Journal of Experimental and Clinical Cancer Research, Vol. 13, No. 1, 1994, p. 61-65.

Research output: Contribution to journalArticle

@article{9e9573601693433fb0313b0a3b79de44,
title = "Enhanced antibody-dependent cell-mediated cytotoxicity of peripheral blood mononuclear cells in patients with germinal cell tumors",
abstract = "The antibody-dependent cell mediated cytotoxicity (K-cell activity) of peripheral blood mononuclear cells from 92 patients with germinal cell tumors and 60 healthy male controls was measured against 0,Rh(D) positive human red blood cells sensitized with anti-D antibody. To determine the maximal K-cell activity the enyzme-like kinetic model of cytotoxicity-in which lysis was measured in presence of target cell excess, was utilized. To avoid variations due to the individual sensitivity of target erythrocytes red blood cells were obtained from a single donor. It was demonstrated that the R-cell activity of patients with germinal cell tumors was significantly enhanced compared to the control group. Patients with clinically detectable tumors showed relevant higher activity than those without detectable tumor. Chemotherapy significantly enhanced the activity of patients in the former group.",
keywords = "ADCC, chemotherapy, germinal cell tumor, K-cell",
author = "R. Kirschner and C. Kulhavi and I. Bodrogi and M. Boki and P. Siklos and T. Bakacs",
year = "1994",
language = "English",
volume = "13",
pages = "61--65",
journal = "Journal of Experimental and Clinical Cancer Research",
issn = "0392-9078",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Enhanced antibody-dependent cell-mediated cytotoxicity of peripheral blood mononuclear cells in patients with germinal cell tumors

AU - Kirschner, R.

AU - Kulhavi, C.

AU - Bodrogi, I.

AU - Boki, M.

AU - Siklos, P.

AU - Bakacs, T.

PY - 1994

Y1 - 1994

N2 - The antibody-dependent cell mediated cytotoxicity (K-cell activity) of peripheral blood mononuclear cells from 92 patients with germinal cell tumors and 60 healthy male controls was measured against 0,Rh(D) positive human red blood cells sensitized with anti-D antibody. To determine the maximal K-cell activity the enyzme-like kinetic model of cytotoxicity-in which lysis was measured in presence of target cell excess, was utilized. To avoid variations due to the individual sensitivity of target erythrocytes red blood cells were obtained from a single donor. It was demonstrated that the R-cell activity of patients with germinal cell tumors was significantly enhanced compared to the control group. Patients with clinically detectable tumors showed relevant higher activity than those without detectable tumor. Chemotherapy significantly enhanced the activity of patients in the former group.

AB - The antibody-dependent cell mediated cytotoxicity (K-cell activity) of peripheral blood mononuclear cells from 92 patients with germinal cell tumors and 60 healthy male controls was measured against 0,Rh(D) positive human red blood cells sensitized with anti-D antibody. To determine the maximal K-cell activity the enyzme-like kinetic model of cytotoxicity-in which lysis was measured in presence of target cell excess, was utilized. To avoid variations due to the individual sensitivity of target erythrocytes red blood cells were obtained from a single donor. It was demonstrated that the R-cell activity of patients with germinal cell tumors was significantly enhanced compared to the control group. Patients with clinically detectable tumors showed relevant higher activity than those without detectable tumor. Chemotherapy significantly enhanced the activity of patients in the former group.

KW - ADCC

KW - chemotherapy

KW - germinal cell tumor

KW - K-cell

UR - http://www.scopus.com/inward/record.url?scp=0028366066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028366066&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0028366066

VL - 13

SP - 61

EP - 65

JO - Journal of Experimental and Clinical Cancer Research

JF - Journal of Experimental and Clinical Cancer Research

SN - 0392-9078

IS - 1

ER -