Enhanced activity of galactono-1,4-lactone dehydrogenase and ascorbate-glutathione cycle in mitochondria from complex III deficient Arabidopsis

Laura Zsigmond, Bálint Tomasskovics, Veronika Deák, Gábor Rigó, L. Szabados, G. Bánhegyi, A. Szarka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The mitochondrial antioxidant homeostasis was investigated in Arabidopsis ppr40-1 mutant, which presents a block of electron flow at complex III. The activity of the ascorbate biosynthetic enzyme, l-galactono-1,4-lactone dehydrogenase (EC 1.3.2.3) (GLDH) was elevated in mitochondria isolated from mutant plants. In addition increased activities of the enzymes of Foyer-Halliwell-Asada cycle and elevated glutathione (GSH) level were observed in the mutant mitochondria. Lower ascorbate and ascorbate plus dehydroascorbate contents were detected at both cellular and mitochondrial level. Moreover, the more oxidized mitochondrial redox status of ascorbate in the ppr40-1 mutant indicated that neither the enhanced activity of GLDH nor Foyer-Halliwell-Asada cycle could compensate for the enhanced ascorbate consumption in the absence of a functional respiratory chain.

Original languageEnglish
Pages (from-to)809-815
Number of pages7
JournalPlant Physiology and Biochemistry
Volume49
Issue number8
DOIs
Publication statusPublished - Aug 2011

Fingerprint

galactonolactone dehydrogenase
glutathione dehydrogenase (ascorbate)
Electron Transport Complex III
lactones
Arabidopsis
glutathione
Mitochondria
mitochondria
mutants
Enzymes
Electron Transport
Oxidation-Reduction
Glutathione
Homeostasis
Antioxidants
Electrons
electron transport chain
homeostasis
electrons
enzyme activity

Keywords

  • Ascorbate
  • Ascorbate biosynthesis
  • Foyer-Halliwell-Asada cycle
  • Glutathione
  • Mitochondria
  • Respiratory chain

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Enhanced activity of galactono-1,4-lactone dehydrogenase and ascorbate-glutathione cycle in mitochondria from complex III deficient Arabidopsis. / Zsigmond, Laura; Tomasskovics, Bálint; Deák, Veronika; Rigó, Gábor; Szabados, L.; Bánhegyi, G.; Szarka, A.

In: Plant Physiology and Biochemistry, Vol. 49, No. 8, 08.2011, p. 809-815.

Research output: Contribution to journalArticle

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