Engineering resistance to PVY in different potato cultivars in a marker-free transformation system using a 'shooter mutant' A. tumefaciens

Ágnes Bukovinszki, Zoltán Divéki, Márta Csányi, László Palkovics, Ervin Balázs

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this work, Potato virus Y (PVY) resistant potatoes were generated using an environmentally safe construct. For this purpose, a 'shooter' mutant Agrobacterium-based transformation system was used. The isopentenyl transferase gene (ipt) present on the Ti plasmid of 'shooter' strains enhances shoot regeneration and can be used as a phenotypic selection marker. The introduced marker-free binary vector carried a hairpin construct derived from the coat protein gene of PVY-NTN strain in order to induce gene silencing. Transformation resulted in high regeneration rates (1.4-5.7 shoots per explant). With pre-selection for the ipt+ phenotype the transformation frequency was 24-53%, while without selection 12-28% of the shoots were PCR positive. The presence of the transgene was verified by Southern hybridization. In 16 of 31 challenged transformant lines PVY could be detected neither by RT-PCR nor by back inoculation. A 62.5% of these resistant lines proved to be also ipt-free. This transformation system was reproducible in four potato cultivars, suggesting that it could easily be adapted for other species.

Original languageEnglish
Pages (from-to)459-465
Number of pages7
JournalPlant Cell Reports
Volume26
Issue number4
DOIs
Publication statusPublished - Apr 1 2007

Keywords

  • 'Shooter' mutant Agrobacterium
  • Hairpin construct
  • PVY resistance
  • Potato

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Engineering resistance to PVY in different potato cultivars in a marker-free transformation system using a 'shooter mutant' A. tumefaciens'. Together they form a unique fingerprint.

  • Cite this