Engineering enzyme-driven dynamic behaviour in lipid vesicles

Ylenia Miele, T. Bánsági, Annette F. Taylor, Pasquale Stano, Federico Rossi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

The urea-urease system is a pH dependent enzymatic reaction that was proposed as a convenient model to study pH oscillations in vitro; here, in order to determine the best conditions for oscillations, a two-variable model is used in which acid and substrate, urea, are supplied at rates kh and ks from an external medium to an enzyme-containing compartment. Oscillations were observed between pH 4 and 8. Thus the reaction appears a good candidate for the observation of oscillations in experiments, providing the necessary condition that kh > ks is met. In order to match these conditions, we devised an experimental system where we can ensure the fast transport of acid to the encapsulated urease, compared to that of urea. In particular, by means of the droplet transfer method, we encapsulate the enzyme, together with a suitable pH indicator, in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) lipid membrane, where differential diffusion of H+ and urea is ensured by the different permeability (Pm) of membranes to the two species. Here we present preliminary tests for the stability of the enzymatic reaction in the presence of lipids and also the successful encapsulation of the enzyme into lipid vesicles.

Original languageEnglish
Title of host publicationCommunications in Computer and Information Science
PublisherSpringer Verlag
Pages197-208
Number of pages12
Volume587
ISBN (Print)9783319326948
DOIs
Publication statusPublished - 2016
Event10th Italian Workshop on Artificial Life and Evolutionary Computation, WIVACE 2015 - Bari, Italy
Duration: Sep 22 2015Sep 25 2015

Publication series

NameCommunications in Computer and Information Science
Volume587
ISSN (Print)18650929

Other

Other10th Italian Workshop on Artificial Life and Evolutionary Computation, WIVACE 2015
CountryItaly
CityBari
Period9/22/159/25/15

Fingerprint

Urea
Lipids
Enzymes
Acids
Encapsulation
Membranes
Substrates
Experiments

Keywords

  • Enzymatic oscillators
  • Lipid vesicles
  • pH oscillators
  • Urea-urease reaction

ASJC Scopus subject areas

  • Computer Science(all)

Cite this

Miele, Y., Bánsági, T., Taylor, A. F., Stano, P., & Rossi, F. (2016). Engineering enzyme-driven dynamic behaviour in lipid vesicles. In Communications in Computer and Information Science (Vol. 587, pp. 197-208). (Communications in Computer and Information Science; Vol. 587). Springer Verlag. https://doi.org/10.1007/978-3-319-32695-5_18

Engineering enzyme-driven dynamic behaviour in lipid vesicles. / Miele, Ylenia; Bánsági, T.; Taylor, Annette F.; Stano, Pasquale; Rossi, Federico.

Communications in Computer and Information Science. Vol. 587 Springer Verlag, 2016. p. 197-208 (Communications in Computer and Information Science; Vol. 587).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Miele, Y, Bánsági, T, Taylor, AF, Stano, P & Rossi, F 2016, Engineering enzyme-driven dynamic behaviour in lipid vesicles. in Communications in Computer and Information Science. vol. 587, Communications in Computer and Information Science, vol. 587, Springer Verlag, pp. 197-208, 10th Italian Workshop on Artificial Life and Evolutionary Computation, WIVACE 2015, Bari, Italy, 9/22/15. https://doi.org/10.1007/978-3-319-32695-5_18
Miele Y, Bánsági T, Taylor AF, Stano P, Rossi F. Engineering enzyme-driven dynamic behaviour in lipid vesicles. In Communications in Computer and Information Science. Vol. 587. Springer Verlag. 2016. p. 197-208. (Communications in Computer and Information Science). https://doi.org/10.1007/978-3-319-32695-5_18
Miele, Ylenia ; Bánsági, T. ; Taylor, Annette F. ; Stano, Pasquale ; Rossi, Federico. / Engineering enzyme-driven dynamic behaviour in lipid vesicles. Communications in Computer and Information Science. Vol. 587 Springer Verlag, 2016. pp. 197-208 (Communications in Computer and Information Science).
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