Engineering Dictyostelium discoideum myosin II for the introduction of site-specific fluorescence probes

Stuart Wakelin, Paul B. Conibear, Robert J. Woolley, David N. Floyd, Clive R. Bagshaw, Mihály Kovács, András Málnási-Csizmadia

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Dictyostelium discoideum is a useful host for the production of constructs for the analysis of structure-function relationships of myosin. Here we describe the use of myosin II constructs containing a single tryptophan residue, at different locations, for probing events at the nucleotide binding site, the relay loop and the communication path between them. GFP fusions have also been expressed at the N- and C-termini of the myosin motor to provide sensitive probes of the actomyosin dissociation reaction in microscope-based kinetic assays. We report on the fluorescence anisotropy of these constructs in the context of their use as resonance energy transfer probes.

Original languageEnglish
Pages (from-to)673-683
Number of pages11
JournalJournal of Muscle Research and Cell Motility
Volume23
Issue number7-8
DOIs
Publication statusPublished - Dec 1 2002

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ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cell Biology

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