Endotoxin-induced inhibition of mesenteric vasodilator responses to acetylcholine, bradykinin, and post-occlusion hyperemia in anesthetized rats

Zahra Fatehi-Hassanabad, J. Parratt, Brian L. Furman

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Abstract

The effects of endotoxin (20 mg kg-1 i.p.) on the mesenteric vascular responses to acetylcholine, bradykinin, sodium nitroprusside, and to transient occlusion of the superior mesenteric artery were examined in rats anesthetized with pentobarbitone. Mesenteric vasodilator responses to close arterial injections of acetylcholine and bradykinin were reduced at 1.5 h after endotoxin and almost abolished by 4 h; responses to sodium nitroprusside were unaffected. Occlusion of the superior mesenteric artery for 30, 60, or 120 s produced, on release of the occlusion, a time-dependent vasodilator response in the mesenteric circulation (post-occlusion hyperemia). This hyperemia was markedly reduced by nitro-L-arginine methyl ester (L-NAME); L-NAME did not modify acetylcholine-induced vasodilatation. Endotoxin-pretreatment did not modify mesenteric post-occlusion hyperemia 1.5 h after administration but markedly reduced the response by 2.5 h. The administration of L-NAME to endotoxin-treated rats did not further attenuate the hyperemic responses. Mesenteric vasoconstrictor responses to phenylephrine were not modified by endotoxin, although systemic pressor responses to this agent were impaired. We concluded that endotoxin impairs endothelium and nitric oxide-dependent vasodilator responses in the mesenteric circulation.

Original languageEnglish
Pages (from-to)371-376
Number of pages6
JournalShock
Volume6
Issue number5
Publication statusPublished - Nov 1996

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Hyperemia
Bradykinin
Vasodilator Agents
Endotoxins
Acetylcholine
NG-Nitroarginine Methyl Ester
Splanchnic Circulation
Superior Mesenteric Artery
Nitroprusside
Phenylephrine
Vasoconstrictor Agents
Pentobarbital
Vasodilation
Endothelium
Blood Vessels
Nitric Oxide
Injections

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

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Endotoxin-induced inhibition of mesenteric vasodilator responses to acetylcholine, bradykinin, and post-occlusion hyperemia in anesthetized rats. / Fatehi-Hassanabad, Zahra; Parratt, J.; Furman, Brian L.

In: Shock, Vol. 6, No. 5, 11.1996, p. 371-376.

Research output: Contribution to journalArticle

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