Endothelin-A receptor antagonist BQ-485 protects against intraocular pressure spike induced by laser trabeculoplasty in the rabbit

G. Holló, Péter Kóthy, P. Lakatos, P. Vargha

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: We have previously shown in rabbits that the intraocular pressure (IOP) spike caused by argon laser trabeculoplasty (ALT) is associated with an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour. In this study we investigated whether pretreatment with an ETA receptor antagonist (BQ-485) protects against the pressure spike induced by ALT, in the rabbit model. Methods: Under general anaesthesia, 10 μl of 10-5 M BQ-485 was injected into the anterior chamber of the right eye, and 10 μl of balanced salt solution (BSS) into the contralateral anterior chamber, for 12 pigmented rabbits. Five minutes later ALT (1,000 mW, 0.1 s, 100 spots over 360° focused on the iris pillars) was performed on both eyes of each animal. IOP was measured before the injections (baseline value), and also 30 min afterwards using a Tono-Pen XL tonometer. Immediately after the second IOP measurement aqueous humour was aspirated for measurement of ET-1 concentration. Results: The baseline IOP (mean ± SD) was 8.08 ± 1.73 mm Hg and 7.92 ± 1.78 mm Hg for the right and left eyes, respectively (Duncan test, p > 0.05). At 25 min after ALT the IOP of the BQ-485-pretreated right eyes remained unchanged (7.83 ± 2.44 mm Hg, p > 0.05) but the IOP of the BSS-pretreated left eyes at 30 min increased significantly to 10.67 ± 4.70 mm Hg (p <0.05 for comparisons both with the corresponding baseline value for the same eye, and with the IOP of the contralateral eye at 30 min). There was no difference in aqueous humour ET-1 concentration between the corresponding right and left eyes (paired t test, p > 0.05). Conclusion: Intracameral BQ-485 pretreatment protected against the ALT-induced acute IOP elevation, but did not influence the laser-induced ET-1 release. This suggests that ETA receptor antagonists may potentially have a therapeutic role in the prevention of laser-induced IOP spikes.

Original languageEnglish
Pages (from-to)459-462
Number of pages4
JournalOphthalmologica
Volume216
Issue number6
DOIs
Publication statusPublished - 2002

Fingerprint

Trabeculectomy
Intraocular Pressure
Lasers
Argon
Rabbits
Endothelin-1
Aqueous Humor
Anterior Chamber
Salts
BQ 485
Endothelin A Receptor Antagonists
Iris
General Anesthesia
Pressure
Injections

Keywords

  • Argon laser trabeculoplasty
  • BQ-485
  • Endothelin 1
  • Endothelin antagonists
  • Glaucoma
  • Intraocular pressure
  • Rabbit

ASJC Scopus subject areas

  • Ophthalmology

Cite this

@article{c376ebfd222547ce9ef786f2f5668172,
title = "Endothelin-A receptor antagonist BQ-485 protects against intraocular pressure spike induced by laser trabeculoplasty in the rabbit",
abstract = "Purpose: We have previously shown in rabbits that the intraocular pressure (IOP) spike caused by argon laser trabeculoplasty (ALT) is associated with an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour. In this study we investigated whether pretreatment with an ETA receptor antagonist (BQ-485) protects against the pressure spike induced by ALT, in the rabbit model. Methods: Under general anaesthesia, 10 μl of 10-5 M BQ-485 was injected into the anterior chamber of the right eye, and 10 μl of balanced salt solution (BSS) into the contralateral anterior chamber, for 12 pigmented rabbits. Five minutes later ALT (1,000 mW, 0.1 s, 100 spots over 360° focused on the iris pillars) was performed on both eyes of each animal. IOP was measured before the injections (baseline value), and also 30 min afterwards using a Tono-Pen XL tonometer. Immediately after the second IOP measurement aqueous humour was aspirated for measurement of ET-1 concentration. Results: The baseline IOP (mean ± SD) was 8.08 ± 1.73 mm Hg and 7.92 ± 1.78 mm Hg for the right and left eyes, respectively (Duncan test, p > 0.05). At 25 min after ALT the IOP of the BQ-485-pretreated right eyes remained unchanged (7.83 ± 2.44 mm Hg, p > 0.05) but the IOP of the BSS-pretreated left eyes at 30 min increased significantly to 10.67 ± 4.70 mm Hg (p <0.05 for comparisons both with the corresponding baseline value for the same eye, and with the IOP of the contralateral eye at 30 min). There was no difference in aqueous humour ET-1 concentration between the corresponding right and left eyes (paired t test, p > 0.05). Conclusion: Intracameral BQ-485 pretreatment protected against the ALT-induced acute IOP elevation, but did not influence the laser-induced ET-1 release. This suggests that ETA receptor antagonists may potentially have a therapeutic role in the prevention of laser-induced IOP spikes.",
keywords = "Argon laser trabeculoplasty, BQ-485, Endothelin 1, Endothelin antagonists, Glaucoma, Intraocular pressure, Rabbit",
author = "G. Holl{\'o} and P{\'e}ter K{\'o}thy and P. Lakatos and P. Vargha",
year = "2002",
doi = "10.1159/000067547",
language = "English",
volume = "216",
pages = "459--462",
journal = "Ophthalmologica",
issn = "0030-3755",
publisher = "S. Karger AG",
number = "6",

}

TY - JOUR

T1 - Endothelin-A receptor antagonist BQ-485 protects against intraocular pressure spike induced by laser trabeculoplasty in the rabbit

AU - Holló, G.

AU - Kóthy, Péter

AU - Lakatos, P.

AU - Vargha, P.

PY - 2002

Y1 - 2002

N2 - Purpose: We have previously shown in rabbits that the intraocular pressure (IOP) spike caused by argon laser trabeculoplasty (ALT) is associated with an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour. In this study we investigated whether pretreatment with an ETA receptor antagonist (BQ-485) protects against the pressure spike induced by ALT, in the rabbit model. Methods: Under general anaesthesia, 10 μl of 10-5 M BQ-485 was injected into the anterior chamber of the right eye, and 10 μl of balanced salt solution (BSS) into the contralateral anterior chamber, for 12 pigmented rabbits. Five minutes later ALT (1,000 mW, 0.1 s, 100 spots over 360° focused on the iris pillars) was performed on both eyes of each animal. IOP was measured before the injections (baseline value), and also 30 min afterwards using a Tono-Pen XL tonometer. Immediately after the second IOP measurement aqueous humour was aspirated for measurement of ET-1 concentration. Results: The baseline IOP (mean ± SD) was 8.08 ± 1.73 mm Hg and 7.92 ± 1.78 mm Hg for the right and left eyes, respectively (Duncan test, p > 0.05). At 25 min after ALT the IOP of the BQ-485-pretreated right eyes remained unchanged (7.83 ± 2.44 mm Hg, p > 0.05) but the IOP of the BSS-pretreated left eyes at 30 min increased significantly to 10.67 ± 4.70 mm Hg (p <0.05 for comparisons both with the corresponding baseline value for the same eye, and with the IOP of the contralateral eye at 30 min). There was no difference in aqueous humour ET-1 concentration between the corresponding right and left eyes (paired t test, p > 0.05). Conclusion: Intracameral BQ-485 pretreatment protected against the ALT-induced acute IOP elevation, but did not influence the laser-induced ET-1 release. This suggests that ETA receptor antagonists may potentially have a therapeutic role in the prevention of laser-induced IOP spikes.

AB - Purpose: We have previously shown in rabbits that the intraocular pressure (IOP) spike caused by argon laser trabeculoplasty (ALT) is associated with an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour. In this study we investigated whether pretreatment with an ETA receptor antagonist (BQ-485) protects against the pressure spike induced by ALT, in the rabbit model. Methods: Under general anaesthesia, 10 μl of 10-5 M BQ-485 was injected into the anterior chamber of the right eye, and 10 μl of balanced salt solution (BSS) into the contralateral anterior chamber, for 12 pigmented rabbits. Five minutes later ALT (1,000 mW, 0.1 s, 100 spots over 360° focused on the iris pillars) was performed on both eyes of each animal. IOP was measured before the injections (baseline value), and also 30 min afterwards using a Tono-Pen XL tonometer. Immediately after the second IOP measurement aqueous humour was aspirated for measurement of ET-1 concentration. Results: The baseline IOP (mean ± SD) was 8.08 ± 1.73 mm Hg and 7.92 ± 1.78 mm Hg for the right and left eyes, respectively (Duncan test, p > 0.05). At 25 min after ALT the IOP of the BQ-485-pretreated right eyes remained unchanged (7.83 ± 2.44 mm Hg, p > 0.05) but the IOP of the BSS-pretreated left eyes at 30 min increased significantly to 10.67 ± 4.70 mm Hg (p <0.05 for comparisons both with the corresponding baseline value for the same eye, and with the IOP of the contralateral eye at 30 min). There was no difference in aqueous humour ET-1 concentration between the corresponding right and left eyes (paired t test, p > 0.05). Conclusion: Intracameral BQ-485 pretreatment protected against the ALT-induced acute IOP elevation, but did not influence the laser-induced ET-1 release. This suggests that ETA receptor antagonists may potentially have a therapeutic role in the prevention of laser-induced IOP spikes.

KW - Argon laser trabeculoplasty

KW - BQ-485

KW - Endothelin 1

KW - Endothelin antagonists

KW - Glaucoma

KW - Intraocular pressure

KW - Rabbit

UR - http://www.scopus.com/inward/record.url?scp=0036965271&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036965271&partnerID=8YFLogxK

U2 - 10.1159/000067547

DO - 10.1159/000067547

M3 - Article

VL - 216

SP - 459

EP - 462

JO - Ophthalmologica

JF - Ophthalmologica

SN - 0030-3755

IS - 6

ER -