Endothelial function studies in pulmonary vascular disease: determination of angiotensin converting enzyme activity in humans (review).

Attila Cziráki, Iván G. Horváth, Lajos Papp

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Angiotensin converting enzyme (ACE, kininase II) is an endothelial luminal ectoenzyme. The majority of ACE has been found on the pulmonary capillary endothelium (PCE). Pulmonary capillary endothelium-bound (PCEB)-ACE has been extensively studied by means of indicator dilution techniques in animals and man. We have recently developed and applied methodologies for assaying pulmonary capillary endothelium-bound (PCEB) angiotensin converting enzyme (ACE) activity in man. This article provides a summary of our studies in human subjects utilizing similar methodology. Trace amounts of the specific ACE substrate, (3)H-benzoyl-Phe-Ala-Pro ((3)H-BPAP; 40 microCi or 2 nmol), was injected as a bolus into the subclavian vein of patients and immediately blood was withdrawn from a radial arterial catheter. Plasma concentrations of surviving substrate and product ((3)H-benzoyl-Phe) were estimated and BPAP utilization was calculated during a single transpulmonary passage. To investigate the PCE in this manner we tested the hypothesis that PCEB-ACE is depressed in patients diagnosed with acute lung injury and estimated interaction of an ACE inhibitor (enalaprilat) with PCE in human subjects. An inverse correlation was found between the pulmonary endothelium-bound ACE activity (v) and the lung injury score (r=0.379; p

Original languageEnglish
Pages (from-to)317-325
Number of pages9
JournalInternational Journal of Molecular Medicine
Volume9
Issue number3
Publication statusPublished - Mar 2002

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Peptidyl-Dipeptidase A
Vascular Diseases
Human Activities
Lung Diseases
Vascular Endothelium
Lung
Enalaprilat
Subclavian Vein
Indicator Dilution Techniques
Acute Lung Injury
Lung Injury
Angiotensin-Converting Enzyme Inhibitors
Endothelium
Catheters

ASJC Scopus subject areas

  • Genetics

Cite this

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