Endothelial cells cultured from human brain microvessels produce complement proteins factor H, factor B, C1 inhibitor, and C4

Monika Vastag, Judit Skopál, Judit Kramer, Krasimir Kolev, Zoltán Vokó, Éva Csonka, Raymund Machovich, Zoltán Nagy

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The inflammatory mediators, cytokines and complement proteins are believed to regulate the sequential events during the development. of lesions secondary to ischaemia and reperfusion. The endothelial cell monolayer of the brain microvasculature is the critical interface between the blood-borne mediators and brain tissue. The involvement of these cells in complement production and regulation has not been well documented. In the present study, expression of complement proteins (C1 inhibitor, factor H, factor B, C4) by cultured endothelial cells obtained from human brain microvessels has been characterized. Interferon γ upregulates the production of all the complement factors studied. Serine proteases, plasmin and miniplasmin induce the expression of C4, decrease the level of ELISA detectable C1 inhibitor, and do not affect the production of factors H and B. These data indicate that complement proteins are expressed locally by the brain microvessels, and may modulate the inflammatory responses of brain tissue.

Original languageEnglish
Pages (from-to)5-13
Number of pages9
JournalImmunobiology
Volume199
Issue number1
DOIs
Publication statusPublished - Jul 1998

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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