Endoscopic collection of porcine embryos

K. P. Brüssow, J. Rátky

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

At present only surgical methods are available for the recovery of porcine embryos on a large scale. The objective of this study was to develop a minimally-invasive technique for embryo collection by means of trans-abdominal endoscopy. A total of 28 pubertal gilts was synchronized for ovulation and embryos were recovered at day 5 with a five-point endoscopy. The uterine wall was punctured 15 cm caudal of the utero-tubal-junction (UTJ) and a Rüsch-Gold balloon catheterR (12 Ch.) was inserted into the uterine lumen. After inflation of the balloon, the uterine horn was flushed with 70 ml of medium. In total, 45 uterine horns of 28 gilts (80.4%) were flushed successfully. A total of 208 ova were collected and the recovery rate was 53.6%. After gaining more experience in three consecutive sessions, the recovery rate increased to 34.8% (23/ 66), 43.6% (51/117) and 65.4% (134/205), respectively. Ten days after recovery the gilts, were slaughtered and no adhesions were found. However, protrusions of the endometrium were recorded in the absence of sutures being used to close the uterine wall.

Original languageEnglish
Pages (from-to)711-715
Number of pages5
JournalReproduction in Domestic Animals
Volume31
Issue number6
Publication statusPublished - Dec 1996

Fingerprint

gilts
embryo (animal)
Swine
Embryonic Structures
endoscopy
Endoscopy
swine
inflation
endometrium
Economic Inflation
sutures
Endometrium
Ovulation
ova
Gold
gold
Sutures
Ovum
adhesion
ovulation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • veterinary(all)

Cite this

Endoscopic collection of porcine embryos. / Brüssow, K. P.; Rátky, J.

In: Reproduction in Domestic Animals, Vol. 31, No. 6, 12.1996, p. 711-715.

Research output: Contribution to journalArticle

Brüssow, K. P. ; Rátky, J. / Endoscopic collection of porcine embryos. In: Reproduction in Domestic Animals. 1996 ; Vol. 31, No. 6. pp. 711-715.
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