Endonuclease-free, protoplast-forming enzyme preparation and its application in fungal transformation

Mátyás Mink, Hans Joachim Höltke, Christoph Kessler, Lajos Ferenczy

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An endonuclease-free, protoplast-forming enzyme complex was prepared from the "snail enzyme." The purified preparation has high protoplast-forming activity comparable to the crude enzyme complex without destroying circular plasmid DNA. Furthermore, a higher transformation rate was achieved by the application of the endonuclease-free enzyme complex in both yeast and filamentous fungal vectorhost systems.

Original languageEnglish
Pages (from-to)612-615
Number of pages4
JournalEnzyme and Microbial Technology
Volume12
Issue number8
DOIs
Publication statusPublished - Aug 1990

    Fingerprint

Keywords

  • A. nidulans
  • Protoplast-forming enzyme
  • S. cerevisiae
  • transformation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this