Endochitinase gene-based phylogenetic analysis of Trichoderma

Elke Lieckfeldt, Yolaine Cavignac, C. Fekete, Thomas Börner

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

DNA sequences of the single-copy gene coding for the 42 kDa endochitinase enzyme (EC 3.2.1.14) were used for phylogenetic analysis in Trichoderma. A set of 12 primers was developed and the entire gene was sequenced for 16 strains, and nucleotide and deduced amino acid sequences were compared to data from GenBank for additional Trichoderma strains. Analysis of the sequences revealed parsimony informative variation from 2.4 to 43.6% depending on the part of the gene (exons/introns) and the taxonomic level considered. Results are discussed in comparison to previous data from ITS-1 and ITS-2 rDNA sequencing and suggest the 42 kDa endochitinase gene as a potential molecular marker for reconstructing phylogenetic relationships in the genus Trichoderma at species level.

Original languageEnglish
Pages (from-to)7-15
Number of pages9
JournalMicrobiological Research
Volume155
Issue number1
Publication statusPublished - 2000

Fingerprint

Chitinases
Trichoderma
phylogeny
Genes
genes
Nucleic Acid Databases
chitinase
Ribosomal DNA
Introns
exons
Sequence Analysis
introns
Amino Acid Sequence
Exons
amino acid sequences
Nucleotides
sequence analysis
nucleotides
nucleotide sequences
genetic markers

Keywords

  • Endochitinase
  • Hypocrea
  • Molecular phylogeny
  • Trichoderma

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Lieckfeldt, E., Cavignac, Y., Fekete, C., & Börner, T. (2000). Endochitinase gene-based phylogenetic analysis of Trichoderma. Microbiological Research, 155(1), 7-15.

Endochitinase gene-based phylogenetic analysis of Trichoderma. / Lieckfeldt, Elke; Cavignac, Yolaine; Fekete, C.; Börner, Thomas.

In: Microbiological Research, Vol. 155, No. 1, 2000, p. 7-15.

Research output: Contribution to journalArticle

Lieckfeldt, E, Cavignac, Y, Fekete, C & Börner, T 2000, 'Endochitinase gene-based phylogenetic analysis of Trichoderma', Microbiological Research, vol. 155, no. 1, pp. 7-15.
Lieckfeldt, Elke ; Cavignac, Yolaine ; Fekete, C. ; Börner, Thomas. / Endochitinase gene-based phylogenetic analysis of Trichoderma. In: Microbiological Research. 2000 ; Vol. 155, No. 1. pp. 7-15.
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