Endocannabinoid Signaling Mediates Local Dendritic Coordination between Excitatory and Inhibitory Synapses

Hai Yin Hu, Dennis L.H. Kruijssen, Cátia P. Frias, B. Rózsa, Casper C. Hoogenraad, Corette J. Wierenga

Research output: Contribution to journalArticle

Abstract

Dendritic inhibitory synapses are most efficient in modulating excitatory inputs localized on the same dendrite, but it is unknown whether their location is random or regulated. Here, we show that the formation of inhibitory synapses can be directed by excitatory synaptic activity on the same dendrite. We stimulated dendritic spines close to a GABAergic axon crossing by pairing two-photon glutamate uncaging with postsynaptic depolarization in CA1 pyramidal cells. We found that repeated spine stimulation promoted growth of a GABAergic bouton onto the same dendrite. The dendritic feedback signal required postsynaptic activation of DAGL, which produces the endocannabinoid 2-AG, and was mediated by CB1 receptors. We could also induce inhibitory bouton growth by local, brief applications of 2-AG. Our findings reveal a dendritic signaling mechanism to trigger growth of an inhibitory bouton at dendritic locations with strong excitatory synaptic activity, and this mechanism may serve to ensure inhibitory control over clustered excitatory inputs.

Original languageEnglish
Pages (from-to)666-675.e5
JournalCell Reports
Volume27
Issue number3
DOIs
Publication statusPublished - Apr 16 2019

Fingerprint

Endocannabinoids
Dendrites
Synapses
Growth
Cannabinoid Receptor CB1
Dendritic Spines
Pyramidal Cells
Depolarization
Photons
Axons
Glutamic Acid
Spine
Chemical activation
Feedback

Keywords

  • activity-dependent adaptation
  • dendritic computation
  • E/I balance
  • endocannabinoid signaling
  • inhibitory synapses
  • presynaptic boutons
  • synapse formation
  • two-photon glutamate uncaging
  • two-photon microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Endocannabinoid Signaling Mediates Local Dendritic Coordination between Excitatory and Inhibitory Synapses. / Hu, Hai Yin; Kruijssen, Dennis L.H.; Frias, Cátia P.; Rózsa, B.; Hoogenraad, Casper C.; Wierenga, Corette J.

In: Cell Reports, Vol. 27, No. 3, 16.04.2019, p. 666-675.e5.

Research output: Contribution to journalArticle

Hu, Hai Yin ; Kruijssen, Dennis L.H. ; Frias, Cátia P. ; Rózsa, B. ; Hoogenraad, Casper C. ; Wierenga, Corette J. / Endocannabinoid Signaling Mediates Local Dendritic Coordination between Excitatory and Inhibitory Synapses. In: Cell Reports. 2019 ; Vol. 27, No. 3. pp. 666-675.e5.
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