Enantioseparations by high-performance liquid chromatography using macrocyclic glycopeptide-based chiral stationary phases: An overview

István Ilisz, Anita Aranyi, Zoltán Pataj, Antal Péter

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

Since their introduction by Armstrong in 1994, macrocyclic antibiotic-based chiral stationary phases have proven their applicability for the chiral resolution of various types of racemates. The unique structure of macrocyclic glycopeptides and their large variety of interactive sites (e.g., hydrophobic pockets, hydroxyl, amino and carboxyl groups, halogen atoms, aromatic moieties, etc.) are the reason for their wide-ranging selectivity. The commercially available Chirobiotic™ phases, which display complementary characteristics, are capable of separating a broad variety of enantiomeric compounds with good ef fi ciency, good column loadability, high reproducibility, and long-term stability. These are the major reasons for the use of macrocyclic antibiotic-based stationary phases in HPLC enantioseparations. This overview chapter provides a brief summary of general aspects of macrocyclic antibiotic-based chiral stationary phases including their preparation and their application to direct enantioseparations of various racemates focusing on the literature published since 2004.

Original languageEnglish
Title of host publicationChiral Separations
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages137-163
Number of pages27
ISBN (Print)9781627032629
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume970
ISSN (Print)1064-3745

Keywords

  • Chiral stationary phases
  • Chirality
  • Direct separation
  • Enantiomer
  • High-performance liquid chromatography
  • Macrocyclic glycopeptide antibiotics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Ilisz, I., Aranyi, A., Pataj, Z., & Péter, A. (2013). Enantioseparations by high-performance liquid chromatography using macrocyclic glycopeptide-based chiral stationary phases: An overview. In Chiral Separations: Methods and Protocols (pp. 137-163). (Methods in Molecular Biology; Vol. 970). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-263-6_8