Enantiomer separation of disopyramide with capillary electrophoresis using various cyclodextrins

Z. Juvancz, Karin E. Markides, László Jicsinszky

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Enantiomers of disopyramide display different biological actions, and therefore chiral selective analysis is necessary. Fifteen different cyclodextrins (CDs) and CD derivatives were tested as capillary electrophoresis (CE) additives for the chiral separation of disopyramide. Eleven types of CDs showed chiral recognition features and four types had a baseline or close to baseline separation. The best resolution (R(s) = 3.0) was with 15 mM carboxymethylated P-CD (pH 4.9). A sharp decrease in the selectivity of -phosphate (γ-PhoCD) was observed in the pH range of 2-3, indicating a structural change of γ-PhoCD. The enantiomers of disopyramide were separated in its ionized as well as neutral forms using acidic substituted CDs. The results show that the size of the CD cavity can not be used as a guide to estimate chiral separations, suggesting a more complex separation mechanism of these CDs towards disopyramide.

Original languageEnglish
Pages (from-to)1002-1006
Number of pages5
JournalElectrophoresis
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 1997

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Disopyramide
Capillary electrophoresis
Enantiomers
Cyclodextrins
Capillary Electrophoresis
Phosphates

Keywords

  • Capillary electrophoreis
  • Chiral separation
  • Cyclodextrin additives
  • Disopyramide
  • Role of pH

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Enantiomer separation of disopyramide with capillary electrophoresis using various cyclodextrins. / Juvancz, Z.; Markides, Karin E.; Jicsinszky, László.

In: Electrophoresis, Vol. 18, No. 6, 06.1997, p. 1002-1006.

Research output: Contribution to journalArticle

Juvancz, Z. ; Markides, Karin E. ; Jicsinszky, László. / Enantiomer separation of disopyramide with capillary electrophoresis using various cyclodextrins. In: Electrophoresis. 1997 ; Vol. 18, No. 6. pp. 1002-1006.
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