Emetine and macromolecular biosynthesis in mammalian cells

G. Farkas, F. Antoni, M. Staub, P. Piffko

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Abstract

The effect of emetine on protein and RNA synthesis was examined in human tonsillar lymphocytes and in Ehrlich ascites cells. 10-7 M of emetine inhibited protein synthesis by 50% in lymphocytes and by 30% in ascites cells. The inhibition was observed already in the first 10 min and it could not be reversed by washing the cells. The incorporation of (14C) D glucosamine into ascites cells was also inhibited but to a lesser extent than the protein synthesis. At higher concentrations the incorporation of (3H) uridine was also inhibited. 10-3 M emetine exerted 90% inhibition in human tonsillar lymphocytes, whereas in ascites cells the inhibition was 50%. The effect of emetine on RNA synthesis seemed to be reversible in the first 30 min of treatment.

Original languageEnglish
Pages (from-to)63-72
Number of pages10
JournalActa Biochimica et Biophysica Academiae Scientiarum Hungaricae
Volume9
Issue number1-2
Publication statusPublished - Dec 1 1974

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ASJC Scopus subject areas

  • Medicine(all)

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