Emergence of a colistin-resistant KPC-2-producing Klebsiella pneumoniae ST258 clone in Hungary

A. Tóth, I. Damjanova, E. Puskás, L. Jánvári, M. Farkas, A. Dobák, K. Böröcz, J. Pászti

Research output: Contribution to journalArticle

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Abstract

Nine Klebsiella pneumoniae isolates showing non-susceptibility to carbapenems were collected from three centres in the north-eastern region of Hungary. The minimum inhibitory concentrations (MICs) of antibiotics were determined by Etest. The putative production of a carbapenemase was tested by the modified Hodge test. The presence of blaKPC genes was verified by polymerase chain reaction (PCR) and sequencing. Furthermore, molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). All isolates showed extensively drug-resistant (XDR) phenotype, and of these, eight isolates were highly resistant to colistin. The isolates carried blaKPC-2, blaSHV-12, blaTEM-1 and blaSHV-11. PFGE analysis of the nine KPC-2-producing Hungarian ST258 K. pneumoniae isolates, two KPC-2-producing Norwegian ST258 isolates and 33 CTX-M-15-producing ST11 isolates revealed the existence of one genetic cluster at an 88% similarity level. The overall results of the PFGE clustering, MLST and the presence of SHV-11 in both ST11 and ST258 suggest that this is the first hyperepidemic clonal complex of multidrug-resistant K. pneumoniae, probably CC258/CC340, possibly undergoing worldwide spread.

Original languageEnglish
Pages (from-to)765-769
Number of pages5
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume29
Issue number7
DOIs
Publication statusPublished - Jul 2010

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Colistin
Hungary
Pulsed Field Gel Electrophoresis
Klebsiella pneumoniae
Multilocus Sequence Typing
Clone Cells
Disk Diffusion Antimicrobial Tests
Molecular Typing
Carbapenems
Microbial Sensitivity Tests
Cluster Analysis
Anti-Bacterial Agents
Phenotype
Polymerase Chain Reaction
Pharmaceutical Preparations
Genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Medicine(all)

Cite this

Emergence of a colistin-resistant KPC-2-producing Klebsiella pneumoniae ST258 clone in Hungary. / Tóth, A.; Damjanova, I.; Puskás, E.; Jánvári, L.; Farkas, M.; Dobák, A.; Böröcz, K.; Pászti, J.

In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 29, No. 7, 07.2010, p. 765-769.

Research output: Contribution to journalArticle

Tóth, A. ; Damjanova, I. ; Puskás, E. ; Jánvári, L. ; Farkas, M. ; Dobák, A. ; Böröcz, K. ; Pászti, J. / Emergence of a colistin-resistant KPC-2-producing Klebsiella pneumoniae ST258 clone in Hungary. In: European Journal of Clinical Microbiology and Infectious Diseases. 2010 ; Vol. 29, No. 7. pp. 765-769.
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