Emergence and evolution of an international cluster of MDR bacteroides fragilis isolates

József Sóki, Maria Hedberg, Sheila Patrick, Balázs Bálint, Róbert Herczeg, István Nagy, David W. Hecht, E. Nagy, E. Urbán

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objectives: The aim of this study was to examine the antibiotic resistance profiles, antibiotic resistance mechanisms and possible 'clonal' nature of some MDR Bacteroides fragilis strains that simultaneously harboured cfiA, nimB, IS1186 and IS4351. Methods: Antibiotic susceptibilities were determined by Etests and antibiotic resistance genes and different genetic elements were detected by applying PCR methods. The environments of the cfiA and nimB genes were also determined by sequencing. The transferability of the cfiA, nimB and tet(Q) genes was tested by conjugation. The genetic relatedness of the test strains was tested by ERIC-PCR or PFGE. The complete genome sequences of two strains (B. fragilis BF8 and O:21) were determined by next-generation sequencing. Results: Most of the seven B. fragilis strains tested displayed multidrug resistance phenotypes; five strains were resistant to at least five types of antibiotics. Besides the common genetic constitution, ERIC-PCR implied high genetic relatedness. Similarities in some of the antibiotic resistance mechanisms [carbapenems (cfiA) and metronidazole (nimB)] also confirmed their common origin, but some other resistance mechanisms (MLSB [erm(F)] and tetracycline [tet(Q)]) and PFGE typing revealed differences. In B. fragilis BF8 and O:21, erm(F) and tet(X) genes were found with IS4351 borders, thus constituting Tn4351. All the strains were tet(Q) positive and transferred this gene in conjugation experiments, but not the cfiA and nimB genes. Conclusions: An international cluster of MDR B. fragilis strains has been identified and characterized. This 'clone' may have emerged early in the evolution of division II B. fragilis strains, which was suggested by the low-complexity ERIC profiles and differences in the PFGE patterns.

Original languageEnglish
Article numberdkw175
JournalJournal of Antimicrobial Chemotherapy
Volume71
Issue number9
DOIs
Publication statusPublished - May 1 2016

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Bacteroides fragilis
Microbial Drug Resistance
Genes
Polymerase Chain Reaction
vif Genes
Anti-Bacterial Agents
Carbapenems
Constitution and Bylaws
Metronidazole
Multiple Drug Resistance
Tetracycline
Clone Cells
Genome
Phenotype

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Emergence and evolution of an international cluster of MDR bacteroides fragilis isolates. / Sóki, József; Hedberg, Maria; Patrick, Sheila; Bálint, Balázs; Herczeg, Róbert; Nagy, István; Hecht, David W.; Nagy, E.; Urbán, E.

In: Journal of Antimicrobial Chemotherapy, Vol. 71, No. 9, dkw175, 01.05.2016.

Research output: Contribution to journalArticle

Sóki, József ; Hedberg, Maria ; Patrick, Sheila ; Bálint, Balázs ; Herczeg, Róbert ; Nagy, István ; Hecht, David W. ; Nagy, E. ; Urbán, E. / Emergence and evolution of an international cluster of MDR bacteroides fragilis isolates. In: Journal of Antimicrobial Chemotherapy. 2016 ; Vol. 71, No. 9.
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