Embryonic ecdysteroids of Drosophila melanogaster

P. Maróy, Gabrielle Kaufmann, Andreas Dübendorfer

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Titration by RIA of ecdysteroids during embryogenesis of Drosophila shows one single peak at 8 h, i.e. the time of germ-band shortening. An analysis of the ecdysteroids reveals a general shift from low polarity material at 6 h to free ecdysone and (less) 20-hydroxyecdysone at 8 h, and finally to ecdysteroids of mostly higher polarity at 12 h. Adult body walls, when incubated with tritiated 20-hydroxyecdysone, can produce the 22-acetate of this ecdysteroid which then has similar chromatographic properties as ecdysone. We have isolated the putative ecdysone fraction from 8 h embryonic extracts and showed by enzymatic hydrolysis and HPLC that the embryo does not contain 20-hydroxyecdysone-22-acetate, but rather free ecdysone.

Original languageEnglish
Pages (from-to)633-637
Number of pages5
JournalJournal of Insect Physiology
Volume34
Issue number7
DOIs
Publication statusPublished - 1988

Fingerprint

Ecdysteroids
Ecdysone
ecdysone
ecdysteroids
Ecdysterone
Drosophila melanogaster
ecdysterone
Acetates
acetates
enzymatic hydrolysis
titration
integument
shortenings
Drosophila
Embryonic Development
embryo (animal)
Hydrolysis
embryogenesis
Embryonic Structures
High Pressure Liquid Chromatography

Keywords

  • Drosophila
  • ecdysteroid
  • embryo

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Embryonic ecdysteroids of Drosophila melanogaster. / Maróy, P.; Kaufmann, Gabrielle; Dübendorfer, Andreas.

In: Journal of Insect Physiology, Vol. 34, No. 7, 1988, p. 633-637.

Research output: Contribution to journalArticle

Maróy, P. ; Kaufmann, Gabrielle ; Dübendorfer, Andreas. / Embryonic ecdysteroids of Drosophila melanogaster. In: Journal of Insect Physiology. 1988 ; Vol. 34, No. 7. pp. 633-637.
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