Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals

Eric Besson, Stéphane Gastaldi, Emily Bloch, Jacek Zielonka, Monika Zielonka, Balaraman Kalyanaraman, Selma Aslan, Hakim Karoui, Antal Rockenbauer, Olivier Ouari, Micael Hardy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The generation of superoxide radical anion in biological systems is one of the major initiating events in the redox biology of NADPH oxidases and mitochondrial redox signalling. However, the pallette of chemical tools for superoxide detection is very limited, hampering progress in understanding the chemical biology of superoxide. Although EPR spin trapping is regarded as the most rigorous technique for superoxide detection, rapid reduction of the EPR-active superoxide spin adducts to EPR-silent hydroxylamines, or to hydroxyl radical adducts by bioreductants, significantly limits the applicability of this technique in biological systems. To overcome these limitations, in this work, we report the synthesis and characterization of a new mesoporous silica functionalized with a phosphorylated cyclic spin trap (DIPPMPO nitrone). The DIPPMPO-grafted silica is a versatile spin-trap agent enabling the identification of a wide range of carbon or oxygen-centered transient radicals in organic and in aqueous media. Moreover, superoxide was efficiently trapped under in vitro conditions in both cell-free and cellular systems. The generated superoxide adduct exhibited an exceptional half-life of 3.5 h and a resistance toward bioreductant agents such as glutathione for several hours.

Original languageEnglish
Pages (from-to)4194-4203
Number of pages10
JournalAnalyst
Volume144
Issue number14
DOIs
Publication statusPublished - Jul 21 2019

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Fingerprint Dive into the research topics of 'Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals'. Together they form a unique fingerprint.

  • Cite this

    Besson, E., Gastaldi, S., Bloch, E., Zielonka, J., Zielonka, M., Kalyanaraman, B., Aslan, S., Karoui, H., Rockenbauer, A., Ouari, O., & Hardy, M. (2019). Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals. Analyst, 144(14), 4194-4203. https://doi.org/10.1039/c9an00468h