Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals

Eric Besson, Stéphane Gastaldi, Emily Bloch, Jacek Zielonka, Monika Zielonka, Balaraman Kalyanaraman, Selma Aslan, Hakim Karoui, A. Rockenbauer, Olivier Ouari, Micael Hardy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The generation of superoxide radical anion in biological systems is one of the major initiating events in the redox biology of NADPH oxidases and mitochondrial redox signalling. However, the pallette of chemical tools for superoxide detection is very limited, hampering progress in understanding the chemical biology of superoxide. Although EPR spin trapping is regarded as the most rigorous technique for superoxide detection, rapid reduction of the EPR-active superoxide spin adducts to EPR-silent hydroxylamines, or to hydroxyl radical adducts by bioreductants, significantly limits the applicability of this technique in biological systems. To overcome these limitations, in this work, we report the synthesis and characterization of a new mesoporous silica functionalized with a phosphorylated cyclic spin trap (DIPPMPO nitrone). The DIPPMPO-grafted silica is a versatile spin-trap agent enabling the identification of a wide range of carbon or oxygen-centered transient radicals in organic and in aqueous media. Moreover, superoxide was efficiently trapped under in vitro conditions in both cell-free and cellular systems. The generated superoxide adduct exhibited an exceptional half-life of 3.5 h and a resistance toward bioreductant agents such as glutathione for several hours.

Original languageEnglish
Pages (from-to)4194-4203
Number of pages10
JournalAnalyst
Volume144
Issue number14
DOIs
Publication statusPublished - Jul 21 2019

Fingerprint

Spin Trapping
electron spin resonance
Superoxides
Silicon Dioxide
Paramagnetic resonance
trapping
silica
Silica
Biological systems
hydroxyl radical
half life
anion
Negative ions
Oxidation-Reduction
Hydroxylamines
oxygen
Carbon
Oxygen
carbon
NADPH Oxidase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Besson, E., Gastaldi, S., Bloch, E., Zielonka, J., Zielonka, M., Kalyanaraman, B., ... Hardy, M. (2019). Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals. Analyst, 144(14), 4194-4203. https://doi.org/10.1039/c9an00468h

Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals. / Besson, Eric; Gastaldi, Stéphane; Bloch, Emily; Zielonka, Jacek; Zielonka, Monika; Kalyanaraman, Balaraman; Aslan, Selma; Karoui, Hakim; Rockenbauer, A.; Ouari, Olivier; Hardy, Micael.

In: Analyst, Vol. 144, No. 14, 21.07.2019, p. 4194-4203.

Research output: Contribution to journalArticle

Besson, E, Gastaldi, S, Bloch, E, Zielonka, J, Zielonka, M, Kalyanaraman, B, Aslan, S, Karoui, H, Rockenbauer, A, Ouari, O & Hardy, M 2019, 'Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals', Analyst, vol. 144, no. 14, pp. 4194-4203. https://doi.org/10.1039/c9an00468h
Besson E, Gastaldi S, Bloch E, Zielonka J, Zielonka M, Kalyanaraman B et al. Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals. Analyst. 2019 Jul 21;144(14):4194-4203. https://doi.org/10.1039/c9an00468h
Besson, Eric ; Gastaldi, Stéphane ; Bloch, Emily ; Zielonka, Jacek ; Zielonka, Monika ; Kalyanaraman, Balaraman ; Aslan, Selma ; Karoui, Hakim ; Rockenbauer, A. ; Ouari, Olivier ; Hardy, Micael. / Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals. In: Analyst. 2019 ; Vol. 144, No. 14. pp. 4194-4203.
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