Elimination of phenol from the auramine fluorescence staining method for acid fast bacilli

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2 Citations (Scopus)

Abstract

Although the Ziehl-Neelsen stain is probably the best known method for demonstrating acid fast rods in tissue sections, many laboratories use fluorochrome staining for this purpose. This report describes the use of Liquid Organic Cleaner (LOC), a liquid detergent, to replace the toxic phenol in the auramine O fluorescence method for the demonstration of acid-fast bacilli. The modified method yields similar results, but offers several advantages over the method involving phenol as lipophilic agent. Powdered auramine O dissolves better in LOC, the stock solution is utilizable for a longer time, and use of toxic phenol is avoided.

Original languageEnglish
Pages (from-to)241-242
Number of pages2
JournalJournal of Histotechnology
Volume21
Issue number3
DOIs
Publication statusPublished - Jan 1 1998

Keywords

  • Acid-fast bacilli
  • Auramine O
  • Fluorescence
  • Lipophilic agent
  • Mycobacteria

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Medical Laboratory Technology

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