Although the Ziehl-Neelsen stain is probably the best known method for demonstrating acid fast rods in tissue sections, many laboratories use fluorochrome staining for this purpose. This report describes the use of Liquid Organic Cleaner (LOC), a liquid detergent, to replace the toxic phenol in the auramine O fluorescence method for the demonstration of acid-fast bacilli. The modified method yields similar results, but offers several advantages over the method involving phenol as lipophilic agent. Powdered auramine O dissolves better in LOC, the stock solution is utilizable for a longer time, and use of toxic phenol is avoided.
- Acid-fast bacilli
- Auramine O
- Lipophilic agent
ASJC Scopus subject areas
- Medical Laboratory Technology