Elimination of glucose in egg white using immobilized glucose oxidase

C. Sisak, Z. Csanádi, E. Rónay, B. Szajáni

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A commercial glucose oxidase containing catalase (NOVOZYM 771) was immobilized on Amberlite UP 900 anion exchange resin using adsorption and subsequent cross-linking with glutaraldehyde. The maximum protein loading was 33.0 mg g-1 dry support. The activity of the product was 10.2 units g-1 dry solid. The immobilized enzyme was used for the elimination of glucose from egg white in a pilot-scale three-stage fluidized-bed bioreactor with mechanical mixing, equipped with large porous surface for bubble-free transport of oxygen as co-substrate. The biocatalyst packing (10.9 dm3) was uniformly distributed between the stages. The best productivity was achieved using oxygen pressure of 1.5 bar and 2 h residence time at 25 °C.

Original languageEnglish
Pages (from-to)1002-1007
Number of pages6
JournalEnzyme and Microbial Technology
Volume39
Issue number5
DOIs
Publication statusPublished - Sep 4 2006

Fingerprint

Egg White
Glucose Oxidase
Glucose oxidase
Glucose
Anion Exchange Resins
Oxygen
Immobilized Enzymes
Biocatalysts
Glutaral
Bioreactors
Catalase
Fluidized beds
Adsorption
Ion exchange
Negative ions
Enzymes
Resins
Productivity
Proteins
Pressure

Keywords

  • Egg white processing
  • Fluidized-bed bioreactor
  • Glucose elimination
  • Glucose oxidase immobilized

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Elimination of glucose in egg white using immobilized glucose oxidase. / Sisak, C.; Csanádi, Z.; Rónay, E.; Szajáni, B.

In: Enzyme and Microbial Technology, Vol. 39, No. 5, 04.09.2006, p. 1002-1007.

Research output: Contribution to journalArticle

Sisak, C. ; Csanádi, Z. ; Rónay, E. ; Szajáni, B. / Elimination of glucose in egg white using immobilized glucose oxidase. In: Enzyme and Microbial Technology. 2006 ; Vol. 39, No. 5. pp. 1002-1007.
@article{a1439cf9bce648ba973bfa1bd7248d23,
title = "Elimination of glucose in egg white using immobilized glucose oxidase",
abstract = "A commercial glucose oxidase containing catalase (NOVOZYM 771) was immobilized on Amberlite UP 900 anion exchange resin using adsorption and subsequent cross-linking with glutaraldehyde. The maximum protein loading was 33.0 mg g-1 dry support. The activity of the product was 10.2 units g-1 dry solid. The immobilized enzyme was used for the elimination of glucose from egg white in a pilot-scale three-stage fluidized-bed bioreactor with mechanical mixing, equipped with large porous surface for bubble-free transport of oxygen as co-substrate. The biocatalyst packing (10.9 dm3) was uniformly distributed between the stages. The best productivity was achieved using oxygen pressure of 1.5 bar and 2 h residence time at 25 °C.",
keywords = "Egg white processing, Fluidized-bed bioreactor, Glucose elimination, Glucose oxidase immobilized",
author = "C. Sisak and Z. Csan{\'a}di and E. R{\'o}nay and B. Szaj{\'a}ni",
year = "2006",
month = "9",
day = "4",
doi = "10.1016/j.enzmictec.2006.02.010",
language = "English",
volume = "39",
pages = "1002--1007",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Elimination of glucose in egg white using immobilized glucose oxidase

AU - Sisak, C.

AU - Csanádi, Z.

AU - Rónay, E.

AU - Szajáni, B.

PY - 2006/9/4

Y1 - 2006/9/4

N2 - A commercial glucose oxidase containing catalase (NOVOZYM 771) was immobilized on Amberlite UP 900 anion exchange resin using adsorption and subsequent cross-linking with glutaraldehyde. The maximum protein loading was 33.0 mg g-1 dry support. The activity of the product was 10.2 units g-1 dry solid. The immobilized enzyme was used for the elimination of glucose from egg white in a pilot-scale three-stage fluidized-bed bioreactor with mechanical mixing, equipped with large porous surface for bubble-free transport of oxygen as co-substrate. The biocatalyst packing (10.9 dm3) was uniformly distributed between the stages. The best productivity was achieved using oxygen pressure of 1.5 bar and 2 h residence time at 25 °C.

AB - A commercial glucose oxidase containing catalase (NOVOZYM 771) was immobilized on Amberlite UP 900 anion exchange resin using adsorption and subsequent cross-linking with glutaraldehyde. The maximum protein loading was 33.0 mg g-1 dry support. The activity of the product was 10.2 units g-1 dry solid. The immobilized enzyme was used for the elimination of glucose from egg white in a pilot-scale three-stage fluidized-bed bioreactor with mechanical mixing, equipped with large porous surface for bubble-free transport of oxygen as co-substrate. The biocatalyst packing (10.9 dm3) was uniformly distributed between the stages. The best productivity was achieved using oxygen pressure of 1.5 bar and 2 h residence time at 25 °C.

KW - Egg white processing

KW - Fluidized-bed bioreactor

KW - Glucose elimination

KW - Glucose oxidase immobilized

UR - http://www.scopus.com/inward/record.url?scp=33745945312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745945312&partnerID=8YFLogxK

U2 - 10.1016/j.enzmictec.2006.02.010

DO - 10.1016/j.enzmictec.2006.02.010

M3 - Article

AN - SCOPUS:33745945312

VL - 39

SP - 1002

EP - 1007

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 5

ER -