Eleven novel sep genes of Schizosaccharomyces pombe required for efficient cell separation and sexual differentiation

A. Grallert, B. Grallert, E. Zilahi, Z. Szilagyi, M. Sipiczki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Genetic analysis of 20 sterile mutants prone to form hyphae revealed 11 novel ste genes (sep6 to sep16) of Schizosaccharomyces pombe. None of the mutants was completely mycelial. Most mutants formed branching hyphae and showed normal septation. Aberrant septal structures and actin distribution were seen only at 36°C. sep9-307, sep14-576 and sep15-598 showed genetic interactions with sep1-1, a mutation in a forkhead transcription factor homologue. Additional genetic interactions were detected between sep6-194, sep15-598 and cdc16-116, a mutant allele of an anaphase modulator of p34(cdc2), sep9-307 and sep15-598 caused dikaryosis in wee1- background. In mating and sporulation tests, sep6-, sep7-, sep9-, sep1O-, sep11- and sep15- proved to be defective in conjugation only, whereas sep8-, sep13- and sep16- were also defective in meiosis-sporulation. sep12- and sep14- were only partially sterile. All mutants could produce M-factor but sep8-, sep11-, sep15- and sep16- were defective in P-factor production. The mutations in sep8, sep11 and sep16 suppressed the pat1-114-driven meiosis. All mutants were sensitive to the presence of higher concentrations of chloride in the medium and to short heat shocks. The diversity of the mutant phenotypes and the pleiotropic effects of the mutations suggest that these sep genes might act in, or interact with, a multiple overlapping network of regulatory modules.

Original languageEnglish
Pages (from-to)669-686
Number of pages18
JournalYeast
Volume15
Issue number8
DOIs
Publication statusPublished - Jun 15 1999

Fingerprint

Sex Differentiation
Schizosaccharomyces
Cell Separation
Schizosaccharomyces pombe
sexual development
Cell Differentiation
Hyphae
Genes
Meiosis
Forkhead Transcription Factors
mutants
Mutation
Transcription factors
Modulators
Actins
Chlorides
Anaphase
genes
cells
sporulation

Keywords

  • Conjugation
  • Fission yeast
  • Hyphae
  • Pheromone
  • Septum
  • Sporulation
  • Stress response

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Eleven novel sep genes of Schizosaccharomyces pombe required for efficient cell separation and sexual differentiation. / Grallert, A.; Grallert, B.; Zilahi, E.; Szilagyi, Z.; Sipiczki, M.

In: Yeast, Vol. 15, No. 8, 15.06.1999, p. 669-686.

Research output: Contribution to journalArticle

@article{fb74c7f0da8441eab9c9f441a4c31175,
title = "Eleven novel sep genes of Schizosaccharomyces pombe required for efficient cell separation and sexual differentiation",
abstract = "Genetic analysis of 20 sterile mutants prone to form hyphae revealed 11 novel ste genes (sep6 to sep16) of Schizosaccharomyces pombe. None of the mutants was completely mycelial. Most mutants formed branching hyphae and showed normal septation. Aberrant septal structures and actin distribution were seen only at 36°C. sep9-307, sep14-576 and sep15-598 showed genetic interactions with sep1-1, a mutation in a forkhead transcription factor homologue. Additional genetic interactions were detected between sep6-194, sep15-598 and cdc16-116, a mutant allele of an anaphase modulator of p34(cdc2), sep9-307 and sep15-598 caused dikaryosis in wee1- background. In mating and sporulation tests, sep6-, sep7-, sep9-, sep1O-, sep11- and sep15- proved to be defective in conjugation only, whereas sep8-, sep13- and sep16- were also defective in meiosis-sporulation. sep12- and sep14- were only partially sterile. All mutants could produce M-factor but sep8-, sep11-, sep15- and sep16- were defective in P-factor production. The mutations in sep8, sep11 and sep16 suppressed the pat1-114-driven meiosis. All mutants were sensitive to the presence of higher concentrations of chloride in the medium and to short heat shocks. The diversity of the mutant phenotypes and the pleiotropic effects of the mutations suggest that these sep genes might act in, or interact with, a multiple overlapping network of regulatory modules.",
keywords = "Conjugation, Fission yeast, Hyphae, Pheromone, Septum, Sporulation, Stress response",
author = "A. Grallert and B. Grallert and E. Zilahi and Z. Szilagyi and M. Sipiczki",
year = "1999",
month = "6",
day = "15",
doi = "10.1002/(SICI)1097-0061(19990615)15:8<669::AID-YEA411>3.0.CO;2-K",
language = "English",
volume = "15",
pages = "669--686",
journal = "Yeast",
issn = "0749-503X",
publisher = "John Wiley and Sons Ltd",
number = "8",

}

TY - JOUR

T1 - Eleven novel sep genes of Schizosaccharomyces pombe required for efficient cell separation and sexual differentiation

AU - Grallert, A.

AU - Grallert, B.

AU - Zilahi, E.

AU - Szilagyi, Z.

AU - Sipiczki, M.

PY - 1999/6/15

Y1 - 1999/6/15

N2 - Genetic analysis of 20 sterile mutants prone to form hyphae revealed 11 novel ste genes (sep6 to sep16) of Schizosaccharomyces pombe. None of the mutants was completely mycelial. Most mutants formed branching hyphae and showed normal septation. Aberrant septal structures and actin distribution were seen only at 36°C. sep9-307, sep14-576 and sep15-598 showed genetic interactions with sep1-1, a mutation in a forkhead transcription factor homologue. Additional genetic interactions were detected between sep6-194, sep15-598 and cdc16-116, a mutant allele of an anaphase modulator of p34(cdc2), sep9-307 and sep15-598 caused dikaryosis in wee1- background. In mating and sporulation tests, sep6-, sep7-, sep9-, sep1O-, sep11- and sep15- proved to be defective in conjugation only, whereas sep8-, sep13- and sep16- were also defective in meiosis-sporulation. sep12- and sep14- were only partially sterile. All mutants could produce M-factor but sep8-, sep11-, sep15- and sep16- were defective in P-factor production. The mutations in sep8, sep11 and sep16 suppressed the pat1-114-driven meiosis. All mutants were sensitive to the presence of higher concentrations of chloride in the medium and to short heat shocks. The diversity of the mutant phenotypes and the pleiotropic effects of the mutations suggest that these sep genes might act in, or interact with, a multiple overlapping network of regulatory modules.

AB - Genetic analysis of 20 sterile mutants prone to form hyphae revealed 11 novel ste genes (sep6 to sep16) of Schizosaccharomyces pombe. None of the mutants was completely mycelial. Most mutants formed branching hyphae and showed normal septation. Aberrant septal structures and actin distribution were seen only at 36°C. sep9-307, sep14-576 and sep15-598 showed genetic interactions with sep1-1, a mutation in a forkhead transcription factor homologue. Additional genetic interactions were detected between sep6-194, sep15-598 and cdc16-116, a mutant allele of an anaphase modulator of p34(cdc2), sep9-307 and sep15-598 caused dikaryosis in wee1- background. In mating and sporulation tests, sep6-, sep7-, sep9-, sep1O-, sep11- and sep15- proved to be defective in conjugation only, whereas sep8-, sep13- and sep16- were also defective in meiosis-sporulation. sep12- and sep14- were only partially sterile. All mutants could produce M-factor but sep8-, sep11-, sep15- and sep16- were defective in P-factor production. The mutations in sep8, sep11 and sep16 suppressed the pat1-114-driven meiosis. All mutants were sensitive to the presence of higher concentrations of chloride in the medium and to short heat shocks. The diversity of the mutant phenotypes and the pleiotropic effects of the mutations suggest that these sep genes might act in, or interact with, a multiple overlapping network of regulatory modules.

KW - Conjugation

KW - Fission yeast

KW - Hyphae

KW - Pheromone

KW - Septum

KW - Sporulation

KW - Stress response

UR - http://www.scopus.com/inward/record.url?scp=0033564190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033564190&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0061(19990615)15:8<669::AID-YEA411>3.0.CO;2-K

DO - 10.1002/(SICI)1097-0061(19990615)15:8<669::AID-YEA411>3.0.CO;2-K

M3 - Article

C2 - 10392445

AN - SCOPUS:0033564190

VL - 15

SP - 669

EP - 686

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 8

ER -