Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency

I. R. Korponay-Szabó, I. Dahlbom, K. Laurila, S. Koskinen, N. Woolley, J. Partanen, J. Kovács, M. Mäki, T. Hansson

Research output: Contribution to journalArticle

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Abstract

Background: IgA serum autoantibodies against tissue transglutaminase (tTG) have an established diagnostic value in coeliac disease, and high efficacy tests are widely available for their detection. However, serological evaluation of IgA deficient subjects is still difficult. Aims: To evaluate the diagnostic potential of IgG class anti-tTG autoantibodies measured quantitatively using an enzyme linked immunosorbent assay (ELISA) compared with immunofluorescent detection of coeliac autoantibodies. Patients: We tested serum samples from 325 IgA deficient subjects, including 78 patients with coeliac disease, 73 disease controls, and 174 blood donors. Methods: IgG antibodies against human recombinant tTG were measured with an ELISA. IgG antiendomysium antibodies (EMA) were assayed by indirect immunofluorescence on human jejunum and appendix sections. Results: The IgG anti-tTG ELISA had a sensitivity of 98.7% and a specificity of 98.6%, and the correlation with IgG EMA titres was high (r s = 0.91). One coeliac patient, initially negative in all autoantibody tests, displayed both IgG anti-tTG antibodies and IgG EMA during later gluten exposure. IgG anti-tTG antibodies and EMA titres showed significant decreases (p

Original languageEnglish
Pages (from-to)1567-1571
Number of pages5
JournalGut
Volume52
Issue number11
DOIs
Publication statusPublished - Nov 2003

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IgA Deficiency
Celiac Disease
Immunoglobulin G
Autoantibodies
Antibodies
Immunoglobulin A
Enzyme-Linked Immunosorbent Assay
Abdomen
Glutens
Appendix
Jejunum
Indirect Fluorescent Antibody Technique
Blood Donors
Serum
transglutaminase 2
anti-IgG

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Korponay-Szabó, I. R., Dahlbom, I., Laurila, K., Koskinen, S., Woolley, N., Partanen, J., ... Hansson, T. (2003). Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency. Gut, 52(11), 1567-1571. https://doi.org/10.1136/gut.52.11.1567

Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency. / Korponay-Szabó, I. R.; Dahlbom, I.; Laurila, K.; Koskinen, S.; Woolley, N.; Partanen, J.; Kovács, J.; Mäki, M.; Hansson, T.

In: Gut, Vol. 52, No. 11, 11.2003, p. 1567-1571.

Research output: Contribution to journalArticle

Korponay-Szabó, IR, Dahlbom, I, Laurila, K, Koskinen, S, Woolley, N, Partanen, J, Kovács, J, Mäki, M & Hansson, T 2003, 'Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency', Gut, vol. 52, no. 11, pp. 1567-1571. https://doi.org/10.1136/gut.52.11.1567
Korponay-Szabó IR, Dahlbom I, Laurila K, Koskinen S, Woolley N, Partanen J et al. Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency. Gut. 2003 Nov;52(11):1567-1571. https://doi.org/10.1136/gut.52.11.1567
Korponay-Szabó, I. R. ; Dahlbom, I. ; Laurila, K. ; Koskinen, S. ; Woolley, N. ; Partanen, J. ; Kovács, J. ; Mäki, M. ; Hansson, T. / Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency. In: Gut. 2003 ; Vol. 52, No. 11. pp. 1567-1571.
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AU - Koskinen, S.

AU - Woolley, N.

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